RT Journal Article SR Electronic(1) A1 Chang, Ya-Qi A1 Meng, Xue A1 Du, Zhao-Zhong A1 Du, Zong-JunYR 2019 T1 Kineobactrum sediminis gen. nov., sp. nov., isolated from marine sediment JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 8 SP 2395 OP 2400 DO https://doi.org/10.1099/ijsem.0.003492 PB Microbiology Society, SN 1466-5034, AB A novel Gram-stain-negative, rod-shaped marine bacterium, designated strain F02T, was isolated from a marine saltern in Weihai, PR China. The cells of strain F02T were approximately 0.8–1.0×3.0–4.0 µm and motile by means of a polar flagellum. Strain F02T grew optimally at 33–35 °C, pH 7.5 and in the presence of 3.0 % (w/v) NaCl. Strain F02T showed oxidase- and catalase-positive activities. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain F02T belonged to the family Halieaceae and exhibited 16S rRNA gene sequence similarities of 96.6 and 96.4 % to the type strains of Chromatocurvus halotolerans and Parahaliea mediterranea , respectively. The major cellular fatty acids of strain F02T were C18 : 1ω7c, C16 : 1ω7c, C15 : 0 and C18 : 1ω9c. The major polar lipids of strain F02T were phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminolipid. Strain F02T contained Q-8 as the sole respiratory quinone. The genomic DNA G+C content was 58.4 mol%. The genome sequences of strain F02T and Chromatocurvus halotolerans DSM 23344T had an OrthoANI value of 70.5 %, and the average amino acid identity value between the two genomes was 62.6 %. The sequence similarity value between the rpoB genes of strain F02T and Chromatocurvus halotolerans DSM 23344T was 79.5 %. On the basis of polyphasic analysis, strain F02T represents a novel species in a new genus, for which the name Kineobactrum sediminis gen. nov., sp. nov. is proposed. The type strain is F02T (=KCTC 52616T=MCCC 1H00224T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003492