@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003493, author = "Suman, Jachym and Zubrova, Andrea and Rojikova, Katerina and Pechar, Radko and Svec, Pavel and Cajthaml, Tomas and Ulbrich, Pavel and Ridl, Jakub and Strnad, Hynek and Uhlik, Ondrej", title = "Pseudogemmobacter bohemicus gen. nov., sp. nov., a novel taxon from the Rhodobacteraceae family isolated from heavy-metal-contaminated sludge", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2019", volume = "69", number = "8", pages = "2401-2407", doi = "https://doi.org/10.1099/ijsem.0.003493", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003493", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Rhodobacteraceae", keywords = "chemotaxonomic characterization", keywords = "Pseudogemmobacter bohemicus", keywords = "heavy metals", keywords = "percentage of conserved proteins", keywords = "16S rRNA gene analysis", abstract = "The creamy white to beige, aerobic, non-motile, ovoid to rod-shaped, Gram-stain-negative strain, Cd-10T, was isolated from heavy-metal-contaminated sludge from a decantation basin of a heavy metal processing factory based on its ability to tolerate CdCl2 in the cultivation medium. In the reconstruction of its phylogeny based on 16S rRNA gene sequences, strain Cd-10T clustered with species of the genera Gemmobacter , Xinfangfangia, Tabrizicola and Rhodobacter within the family Rhodobacteraceae . Its 16S rRNA gene sequence exhibited 96.32 % pairwise similarity to the type strain of Xinfangfangia soli , 95.3 % to that of Gemmobacter intermedius , followed by Tabrizicola fusiformis (95.10 %), Rhodobacter sediminis (94.88 %), Gemmobacter nectariphilus and Rhodobacter capsulatus (both 94.81 %). The major respiratory quinone was Q-10 accompanied by Q-9, the fatty acid profile consisted predominantly of C18 : 1ω7c, C18 : 0, C16 : 0 and C16 : 1ω7c, the major polar lipids were phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine and diphosphatidylglycerol. An analysis of the percentage of conserved proteins deduced from draft or complete genomic sequences of strain Cd-10T and representatives of its closest relatives suggested that strain Cd-10T is a member of a novel genus within the Rhodobacteraceae family for which we propose the name Pseudogemmobacter. Strain Cd-10T (=DSM 103618T=NCCB 100645T) is the type strain of Pseudogemmobacter bohemicus gen. nov., sp. nov., the type species of the genus Pseudogemmobacter gen. nov.", }