%0 Journal Article %A Blackburn, Michael B. %A Farrar Jr., Robert R. %A Sparks, Michael E. %A Kuhar, Daniel %A Mowery, Joseph D. %A Mitchell, Ashaki %A Gundersen-Rindal, Dawn E. %T Chromobacterium phragmitis sp. nov., isolated from estuarine marshes %D 2019 %J International Journal of Systematic and Evolutionary Microbiology, %V 69 %N 9 %P 2681-2686 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003508 %K neisseriales %K tidal marsh %K Betaproteobacteria %K insecticidal bacteria %I Microbiology Society, %X Thirteen isolates of Gram-stain-negative, motile, violet-pigmented bacteria were isolated from marshes along tidal portions of the Potomac and James rivers in Maryland and Virginia, USA, respectively. 16S rRNA gene sequences and fatty acid analysis revealed a high degree of relatedness among the isolates, and genomic sequencing of two isolates, IIBBL 112-1T and IIBBL 274-1 (from the Potomac and James rivers, respectively), revealed highly similar genomic sequences, with a blast-based average nucleotide identity (ANIb) of ca. 98.7 %. Phylogenetic analysis of 16S rRNA gene sequences suggested that the species most highly related to IIBBL 112-1T were Chromobacterium amazonense , Chromobacterium subtsugae and Chromobacterium sphagni . However, deletion of a 25-nucleotide sequence that may have been horizontally acquired by both IIBBL 112-1T and C. amazonense resulted in a substantially different analysis; in the latter case, the species nearest IIBBL 112-1T were Chromobacterium violaceum , Chromobacterium vaccinii and Chromobacterium piscinae . Whole-genome alignments between either IIBBL 112-1T or IIBBL 274-1 and the type strains of C. vaccinii or C. violaceum resulted in ANIb values in the range of ca. 87 %, while alignment with C. amazonense CBMAI 310T resulted in an ANIb of ca. 83 %. Collectively, these data demonstrate that IIBBL 112-1T and IIBBL 274-1 represent a new taxon within the genus Chromobacterium . We propose the name Chromobacterium phragmitis sp. nov. for this taxon; the type strain is IIBBL 112-1T (=NRRL B-67132T=JCM 31884T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003508