1887

Abstract

Four Gram-stain-positive strains, R7, R11, R19 and R27, were isolated from suan-tsai, a traditional fermented mustard green product of Taiwan. Cells were rod-shaped, non-motile, non-haemolytic, asporogenous, facultatively anaerobic, heterofermentative, and did not exhibit catalase and oxidase activities. Comparative analyses of 16S rRNA, and gene sequences demonstrated that these novel strains were members of the genus . 16S rRNA and the concatenated and gene sequence similarities between strains R7 and R11, and strains R19 and R27 were very high (>99.8 % similarity), respectively. On the basis of 16S rRNA gene sequence similarities, the type strains of (98.5 %), (98.5 %), (98.1 %) and (98.1 %) were the closest neighbours to strains R7 and R11, and the type strains of (98.9 %), (98.4 %), (98.4 %), (98.4 %) and (98.0 %) were the closest neighbours to strains R19 and R27, respectively. The average nucleotide identity values of R7 and R19 with the closely related type strains were 78.9–80.1% and 75.7–80.5 %, respectively. The digital DNA–DNA hybridization values were 22.8–23.6% and 21.0–23.1 %, respectively. Phenotypic and genotypic test results demonstrated that these strains represent two novel species of the genus , for which the name sp. nov. (R7=BCRC 81127=NBRC 113530) and sp. nov. (R19=BCRC 81129=NBRC 113532) are proposed.

Funding
This study was supported by the:
  • Ministry of Economic Affairs, R.O.C. (Award Project No. 106-EC-17-A-22-0525)
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2019-06-14
2024-03-28
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References

  1. Chen YS, Yanagida F, Hsu JS. Isolation and characterization of lactic acid bacteria from suan-tsai (fermented mustard), a traditional fermented food in Taiwan. J Appl Microbiol 2006; 101:125–130 [View Article][PubMed]
    [Google Scholar]
  2. Chao SH, Wu RJ, Watanabe K, Tsai YC. Diversity of lactic acid bacteria in suan-tsai and fu-tsai, traditional fermented mustard products of Taiwan. Int J Food Microbiol 2009; 135:203–210 [View Article][PubMed]
    [Google Scholar]
  3. Chao SH, Kudo Y, Tsai YC, Watanabe K. Lactobacillus futsaii sp. nov., isolated from fu-tsai and suan-tsai, traditional Taiwanese fermented mustard products. Int J Syst Evol Microbiol 2012; 62:489–494 [View Article][PubMed]
    [Google Scholar]
  4. Huang CH, Huang L, Chang MT, Chen KL. Establishment and application of an analytical in-house database (IHDB) for rapid discrimination of Bacillus subtilis group (BSG) using whole-cell MALDI-TOF MS technology. Mol Cell Probes 2016; 30:312–319 [View Article][PubMed]
    [Google Scholar]
  5. Huang CH, Lee FL. Development of novel species-specific primers for species identification of the Lactobacillus casei group based on RAPD fingerprints. J Sci Food Agric 2009; 89:1831–1837 [View Article]
    [Google Scholar]
  6. Guu JR, Wang LT, Hamada M, Wang C, Lin RW et al. . Lactobacillus bambusae sp. nov., isolated from traditional fermented ma bamboo shoots in Taiwan. Int J Syst Evol Microbiol 2018; 68:2424–2430 [View Article][PubMed]
    [Google Scholar]
  7. Naser SM, Thompson FL, Hoste B, Gevers D, Dawyndt P et al. . Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 2005; 151:2141–2150 [View Article][PubMed]
    [Google Scholar]
  8. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410 [View Article][PubMed]
    [Google Scholar]
  9. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. . Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  10. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA et al. . Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  11. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  12. Chor B, Hendy MD, Snir S. Maximum likelihood Jukes-Cantor triplets: analytic solutions. Mol Biol Evol 2006; 23:626–632 [View Article][PubMed]
    [Google Scholar]
  13. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  14. Rzhetsky A, Nei M. A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 1992; 9:945–967
    [Google Scholar]
  15. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993; 10:512–526 [View Article][PubMed]
    [Google Scholar]
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  17. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  18. Klimke W, Agarwala R, Badretdin A, Chetvernin S, Ciufo S et al. The National Center for Biotechnology Information’s Protein Clusters Database. Nucleic Acids Res 2009; 37:D216–D223 [View Article]
    [Google Scholar]
  19. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article][PubMed]
    [Google Scholar]
  20. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
    [Google Scholar]
  21. Kandler O, Weiss N. Genus Lactobacillus Beijerinck 1901, 212AL. In Sneath PHA, Mair NS, Sharpe ME, Holt JG. (editors) Bergey’s Manual of Systematic Bacteriology vol. 2 Baltimore: Williams & Wilkins; 1986 pp. 1209–1234
    [Google Scholar]
  22. Hamada M, Yamamura H, Komukai C, Tamura T, Suzuki K et al. Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium . J Antibiot 2012; 65:427–431 [View Article][PubMed]
    [Google Scholar]
  23. Nozawa Y, Sakai N, Arai K, Kawasaki Y, Harada K. Reliable and sensitive analysis of amino acids in the peptidoglycan of actinomycetes using the advanced Marfey’s method. J Microbiol Methods 2007; 70:306–311 [View Article][PubMed]
    [Google Scholar]
  24. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 1990; 20:16
    [Google Scholar]
  25. Mañes-Lázaro R, Ferrer S, Rodas AM, Urdiain M, Pardo I. Lactobacillus bobalius sp. nov., a lactic acid bacterium isolated from Spanish Bobal grape must. Int J Syst Evol Microbiol 2008; 58:2699–2703 [View Article][PubMed]
    [Google Scholar]
  26. Yoon JH, Kang SS, Mheen TI, Ahn JS, Lee HJ et al. Lactobacillus kimchii sp. nov., a new species from kimchi. Int J Syst Evol Microbiol 2000; 50:1789–1795 [View Article][PubMed]
    [Google Scholar]
  27. Koob J, Jacob F, Wenning M, Hutzler M. Lactobacillus cerevisiae sp. nov., isolated from a spoiled brewery sample. Int J Syst Evol Microbiol 2017; 67:3452–3457 [View Article][PubMed]
    [Google Scholar]
  28. Valcheva R, Korakli M, Onno B, Prévost H, Ivanova I et al. Lactobacillus hammesii sp. nov., isolated from French sourdough. Int J Syst Evol Microbiol 2005; 55:763–767 [View Article][PubMed]
    [Google Scholar]
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