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Volume 70, Issue 4

gen. nov., sp. nov., a new member of the family isolated from a freshwater mesocosm Free

Abstract

A bacterial strain designated FSY-15 was isolated from a freshwater mesocosm in Taiwan and characterised using a polyphasic taxonomic approach. Cells of strain FSY-15 were Gram-negative, aerobic, non-spore forming, non-motile rods and formed orange coloured colonies. Growth occurred at 20–30 °C (optimum, 25 °C), at pH 6–7.5 (optimum, pH 7) and with 0–0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain FSY-15 formed a phylogenetic lineage in the the family . Strain FSY-15 was most closely related to the genera and , and the levels of 16S rRNA gene sequence identity with respect to members of related genera are less than 94.1 %. Strain FSY-15 showed less than 68.8 % average nucleotide identity and less than 24.7 % digital DNA–DNA hybridisation identity compared to the type strains of related genera within the family . The predominant fatty acids were iso-C, Cω and the major hydroxyl fatty acid was iso-C 3-OH. The major isoprenoid quinone was MK-7 and the DNA G+C content was 35.8 mol%. The major polar lipids were phosphatidylethanolamine and several uncharacterised aminophospholipid, aminolipid, phospholipid and lipid. The major polyamine was spermidine. On the basis of the genotypic and phenotypic data, strain FSY-15 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is FSY-15 (=BCRC 81011 =LMG 29732 =KCTC 52445).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacteroidetes , freshwater mesocosm and Sandaracinomonas limnophila
© 2020 IUMS
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2019-06-14
2024-04-19
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Sandaracinomonas limnophila gen. nov., sp. nov., a new member of the family Cytophagaceae isolated from a freshwater mesocosm
Int J Syst Evol Microbiol 70, 2178 (2020); https://doi.org/10.1099/ijsem.0.003532
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