1887

Abstract

A novel endophytic bacterium, designated strain SX2RGS8, was isolated from the surface-sterilized roots of an endangered medicinal plant ( K. M. Shen) collected from Xinjiang, north-western PR China. The taxonomic position of the candidate was investigated using a polyphasic approach. Strain SX2RGS8 was found to be aerobic, Gram-stain-negative, oxidase-negative, catalase-positive and axiolitic-shaped. Strain SX2RGS8 grew at 4–45 °C (optimum, 28 °C), pH 4.0–10.0 (pH 7.0) and in the presence of 0–5 % (w/v) NaCl. The polar lipids detected for strain SX2RGS8 were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, unidentified phosphoglycolipids, an unidentified phospholipid and unidentified lipids. The major respiratory quinone of strain SX2RGS8 was ubiquinone 10 and the major fatty acid was summed feature 8 (Cω7 and/or Cω6). The DNA G+C content was determined to be 66.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate belonged to the family and showed 99.2 % (), 95.5 % () and 95.4 % () similarities to its closest relatives. The isolate contained carotenoids, but no bacteriochlorophyll . On the basis of phenotypic, genotypic and phylogenetic data, strain SX2RGS8 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is SX2RGS8 (=CGMCC 1.16402=KCTC 62090). In addition, Coil . 2016 is proposed to be transferred to this new genus as comb. nov.

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2019-08-01
2024-03-28
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