@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003547, author = "Le Roy, Tiphaine and Van der Smissen, Patrick and Paquot, Adrien and Delzenne, Nathalie and Muccioli, Giulio G. and Collet, Jean-François and Cani, Patrice D.", title = "Dysosmobacter welbionis gen. nov., sp. nov., isolated from human faeces and emended description of the genus Oscillibacter", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "9", pages = "4851-4858", doi = "https://doi.org/10.1099/ijsem.0.003547", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003547", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Clostridial cluster IV", keywords = "Oscillospiraceae", keywords = "human intestinal microbiota", keywords = "Ruminococcaceae", keywords = "human faeces", abstract = "A strictly anaerobic, Gram-stain-negative, non-spore-forming, non-motile, non-pigmented bacterium, strain J115T, was isolated from human faeces. Cells of strain J115T were straight rods, generally 1.8–3.0 µm, but could be up to 18 µm long. Growth occurred below 2 % (w/v) NaCl and 2 % (v/v) bile. Strain J115T produced acid from myo-inositol but not from d-glucose, d-ribose or d-xylose. Butyric acid was the major end-product from myo-inositol. The genomic DNA G+C content was 58.92 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the closest cultivated neighbours of strain J115T were Oscillibacter ruminantium GH1T (95.4 % similarity) and Oscillibacter valericigenes Sjm18-20T (94.1 %). Strain J115T was also related to the not-yet-cultured bacterium Oscillospira guilliermondii (92–93 % similarity). Coherently with the 16S rRNA gene sequence results, the ANI scores don't have units of strain J115T to O. ruminantium GH1T and O. valericigenes Sjm18-20T were 73.37 and 73.24, respectively, while in silico estimations of DNA–DNA hybridization were both 20.4 %, with confidence intervals of 18.2–22.9 % and 18.2–22.8 %, respectively. The major fatty acids were iso-C15 : 0 (24.2 %), C18 : 0 DMA (18.4 %), anteiso-C15 : 0 (15.2 %) and C16 : 0 DMA (7.6 %). No respiratory quinone was detected. Based on phenotypic features and phylogenetic position, it is proposed that this isolate represents a novel species in a new genus, Dysosmobacter welbionis gen. nov., sp. nov. The type strain of Dysosmobacter welbionis is J115T (DSM 106889T=LMG 30601T).", }