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Volume 69, Issue 9

sp. nov., isolated from Chinese traditional pickle Free

Abstract

A Gram-stain-positive bacterial strain, 395-6.2, was isolated from traditional pickle in Heilongjiang Province, PR China. The bacterium was characterised by a polyphasic approach, including 16S rRNA gene sequence analysis, gene sequence analysis, gene sequence analysis, fatty acid methyl ester (FAME) analysis, determination of DNA G+C content, average nucleotide identity (ANI) analysis, DNA–DNA hybridisation (DDH) and an analysis of phenotypic features. Analysis of the 16S rRNA gene sequence showed that strain 395-6.2 was phylogenetically related to , , , , , , , , , , , , , and . Strain 395-6.2 exhibited 95.7–99.4 % 16S rRNA gene sequence similarities, 85.0–94.0 %  gene sequence similarities, 94.2–98.0 %  gene sequence similarities to type strains of phylogenetically related species. ANI and DDH values between strain 395-6.2 and type strains of phylogenetically related species were 77.9–87.1 % and 22.5–33.5 %, respectively. Based upon the data obtained in the present study, a novel species, sp. nov., is proposed and the type strain is 395-6.2 (=CCM 8927=NCIMB 15188=LMG 31179).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): lactic acid bacteria , Lactobacillus , novel species and pickle
© 2019 IUMS
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2019-09-01
2024-04-25
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Lactobacillus huachuanensis sp. nov., isolated from Chinese traditional pickle
Int J Syst Evol Microbiol 69, 2807 (2019); https://doi.org/10.1099/ijsem.0.003563
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