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Volume 69, Issue 9

Description and complete genome sequence of sp. nov., harbouring photosystem and nitrogen-fixation genes Open Access

Abstract

A bacterial strain, designated 39S1MB, isolated from a root nodule of a soybean plant that had been inoculated with root-zone soil of (hog peanut) growing in Canada, was previously characterized and placed in a novel lineage within the genus . The taxonomic status of strain 39S1MB was verified by genomic and phenotypic analyses. Phylogenetic analyses of individual and concatenated protein-encoding gene sequences (, , , and ) placed 39S1MB in a lineage distinct from named species. Data for sequence similarities of concatenated genes relative to type strains of named species supported the phylogenetic data. Average nucleotide identity values of genome sequences (84.5–91.7 %) were well below the threshold value for bacterial species circumscription. Based on these data, OO99 and ERR11 are close relatives of 39S1MB. The complete genome of 39S1MB consists of a single 7.04 Mbp chromosome without a symbiosis island; G+C content is 64.7 mol%. Present in the genome are key photosystem and nitrogen-fixation genes, but not nodulation and type III secretion system genes. Sequence analysis of the nitrogen fixation gene, , placed 39S1MB in a novel lineage distinct from named species. Data for phenotypic tests including growth characteristics and carbon source utilization supported the sequence-based analyses. Based on the data presented here, a novel species with the name sp. nov. is proposed with 39S1MB (=LMG 29934=HAMBI 3680) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bradyrhizobium amphicarpaeae sp. nov. , complete genome sequence , nitrogen fixation genes and photosystem genes
© 2019 Her Majesty the Queen in right of Canada as represented by the Minister of Agriculture
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2019-09-01
2024-04-24
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Description and complete genome sequence of Bradyrhizobium amphicarpaeae sp. nov., harbouring photosystem and nitrogen-fixation genes
Int J Syst Evol Microbiol 69, 2841 (2019); https://doi.org/10.1099/ijsem.0.003569
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