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Volume 69, Issue 9

sp. nov., isolated from the gut microflora of sea bass ( L.) and emended description of the genus Free

Abstract

A Gram-stain-negative, non-flagellated, catalase-positive, oxidase-positive bacterial strain, designated YLY08, was isolated from the gut microflora of sea bass ( L.) collected from the coast of Yuanyao Wharf, Weihai, PR China, and subjected to a polyphasic taxonomic study. Strain YLY08 grew optimally at 28-30 °C, at pH 7.0–7.5 and in the presence of 2.0–3.0 % (w/v) NaCl. Poly--hydroxybutyrate granules were produced. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain YLY08 clustered with the type strain of , with which it exhibited 95.3 % sequence similarity, while the similarity to other genera was below 95.0 %. Genomic analyses, including average nucleotide identity and the digital DNA–DNA hybridization, clearly separated YLY08 from MCCC 1A11863 with values below the thresholds for species delineation. The major cellular fatty acid was summed feature 8 (Cω7 and/or Cω6). The sole respiratory quinone detected was Q-10. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidyldimethylethanolamine, diphosphatidylglycerol, one unidentified aminolipid and one unidentified phospholipid. The genome of strain YLY08, with 38 assembled contigs, was 3.9 Mb long with a G+C content of 59.0 mol%. The results of the phenotypical, phylogenetic and biochemical analyses between the strain YLY08 and the related type strain indicated that this strain represents a novel species in genus within the family , for which the name sp. nov. is proposed. The type strain is YLY08 (=MCCC 1H00318=KCTC 62182).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Oceaniglobus , PHB and Phylogenetic analysis
© 2019 IUMS
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2019-09-01
2024-04-18
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Oceaniglobus ichthyenteri sp. nov., isolated from the gut microflora of sea bass (Dicentrarchus labrax L.) and emended description of the genus Oceaniglobus
Int J Syst Evol Microbiol 69, 2892 (2019); https://doi.org/10.1099/ijsem.0.003573
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