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Volume 69, Issue 10

sp. nov., isolated from hospital sewage Free

Abstract

A novel Gram-negative, obligate aerobic, rod-shaped, motile by one or two polar flagella, non-spore-forming bacterial strain, WCHPs060044, was isolated from the wastewater treatment plant at West China Hospital in Chengdu, PR China. Analysis of its 16S rRNA gene sequence showed that strain WCHPs060044 belonged to the genus with the highest similarity to KL28 (99.73 %), HYS (99.52 %) and CCOS 846 (99.38 %). Phylogenomic analysis based on 107 core gene sequences demonstrated that WCHPs060044 was a member of the group but was distant from all closely related species. Whole-genome comparisons, using average nucleotide identity based on (ANIb) and DNA–DNA hybridization (isDDH), confirmed low genome relatedness to its close phylogenetic neighbours [below the recommended thresholds of 95 % (ANIb) and 70 % (isDDH) for species delineation]. Phenotypic characterization tests showed that the utilization of -arginine, -fucose, -galacturonic, -glucuronic acid, inositol and sorbitol in combination could distinguish this strain from other related species of the genus . Therefore, based on genetic and phenotypic evidence, strain WCHPs060044 represents a novel species, for which the name sp. nov. is proposed. The type strain is WCHPs060044 (GDMCC 1.1396=JCM 32907=CCTCC AB 2018120=CNCTC 7663).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , Genome , hospital sewage and Pseudomonas huaxiensis
© 2019 IUMS
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2019-10-01
2024-04-25
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Pseudomonas huaxiensis sp. nov., isolated from hospital sewage
Int J Syst Evol Microbiol 69, 3281 (2019); https://doi.org/10.1099/ijsem.0.003622
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