1887

Abstract

Strains A31/70, CCUG 58779 and SD129 were Gram-stain-negative, short rods, motile by one polar flagellum and isolated from clinical specimens in Botswana, Sweden and Spain, respectively. The 16S rRNA sequence similarity values grouped them in the phylogenetic group of species. The DNA G+C content ranged from 65.5 to 65.7 mol%. The strains were characterized phenotypically by the API 20NE and Biolog GEN III tests, and chemotaxonomically by their whole-cell MALDI-TOF MS protein profiles and by their fatty acid contents. The absence of the arginine dihydrolase and the hydrolysis of gelatin differentiated these strains from the closest species, . The major fatty acid contents were summed feature 8 (38.6 %), C (22.6 %), summed feature 3 (20.5 %) and C (8.4 %). Multilocus sequence analysis with three housekeeping gene sequences (, and 16S rRNA) together with whole-genome comparisons indicated that these strains cluster together in the phylogenetic analysis and their similarity values were lower than the thresholds established for species in the genus . These results permit us to conclude that strains A31/70, CCUG 58779 and SD129 belong to a novel species in the genus for which the name sp. nov. is proposed. The type strain is A31/70 (=CECT 9981=CCUG 73638).

Keyword(s): nosocomial and Pseudomonas

Erratum

An erratum has been published for this content:
Corrigendum: sp. nov., isolated from clinical specimens
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003628
2019-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/11/3392.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003628&mimeType=html&fmt=ahah

References

  1. Mulet M, Lalucat J, García-Valdés E. DNA sequence-based analysis of the Pseudomonas species. Environ Microbiol 2010; 12:1513–1530 [View Article][PubMed]
    [Google Scholar]
  2. Azhar EI, Papadioti A, Bibi F, Ashshi AM, Raoult D et al. 'Pseudomonas saudiphocaensis' sp. nov., a new bacterial species isolated from currency notes collected during the Hajj pilgrimage in 2012 at Makkah, Saudi Arabia. New Microbes New Infect 2017; 15:131–133 [View Article][PubMed]
    [Google Scholar]
  3. Parte AC. LPSN - List of prokaryotic names with standing in nomenclature (bacterio.net), 20 years on. Int J Syst Evol Microbiol 2018; 68:1825–1829 [View Article][PubMed]
    [Google Scholar]
  4. Zhang L, Pan Y, Wang K, Zhang X, Zhang S et al. Pseudomonas songnenensis sp. nov., isolated from saline and alkaline soils in Songnen Plain, China. Antonie Van Leeuwenhoek 2015; 107:711–721 [View Article][PubMed]
    [Google Scholar]
  5. Zou Y, He S, Sun Y, Zhang X, Liu Y et al. Pseudomonas urumqiensis sp. nov., isolated from rhizosphere soil of Alhagi sparsifolia . Int J Syst Evol Microbiol 2019; 69:1760–1766 [View Article][PubMed]
    [Google Scholar]
  6. Lalucat J, Bennasar A, Bosch R, García-Valdés E, Palleroni NJ. Biology of Pseudomonas stutzeri . Microbiol Mol Biol Rev 2006; 70:510–547 [View Article][PubMed]
    [Google Scholar]
  7. Mulet M, Gomila M, Ramírez A, Cardew S, Moore ER et al. Uncommonly isolated clinical Pseudomonas: identification and phylogenetic assignation. Eur J Clin Microbiol Infect Dis 2017; 36:351–359 [View Article][PubMed]
    [Google Scholar]
  8. Scotta C, Mulet M, Sánchez D, Gomila M, Ramírez A et al. Identification and genomovar assignation of clinical strains of Pseudomonas stutzeri . Eur J Clin Microbiol Infect Dis 2012; 31:2133–2139 [View Article][PubMed]
    [Google Scholar]
  9. Holmes B. Identification and distribution of Pseudomonas stutzeri in clinical material. J Appl Bacteriol 1986; 60:401–411 [View Article][PubMed]
    [Google Scholar]
  10. Jukes T, Cantor C. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolism New York: Academic Press; 1969 pp. 21–132
    [Google Scholar]
  11. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  12. Nei M, Kumar S. Molecular Evolution and Phylogenetics New York: Oxford University Press; 2000
    [Google Scholar]
  13. Palleroni NJ. Pseudomonas. In Brenner DJ, Krieg NR, Staley JT. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 2 New York: Springer; 2005 pp. 323–379
    [Google Scholar]
  14. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article][PubMed]
    [Google Scholar]
  15. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
    [Google Scholar]
  16. Miller JH. Experiments in molecular genetics. In Cold Spring Harbor Laboratory New York: Cold Spring Harbor; 1972
    [Google Scholar]
  17. Lalucat J. Analysis of refractile (R) bodies. In Mayer E. (editor) Methods in Microbiology –Electron Microscopy in Microbiology vol. 20 London: Academic Press; 1988 pp. 79–90
    [Google Scholar]
  18. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  19. MIDI Sherlock Microbial Identification System Operating Manual, version 6 1. Newark, DE: MIDI Inc; 2008
    [Google Scholar]
  20. Sánchez D, Mulet M, Rodríguez AC, David Z, Lalucat J et al. Pseudomonas aestusnigri sp. nov., isolated from crude oil-contaminated intertidal sand samples after the prestige oil spill. Syst Appl Microbiol 2014; 37:89–94 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003628
Loading
/content/journal/ijsem/10.1099/ijsem.0.003628
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Supplementary File 2

Supplementary File 3

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error