CD55 is a key complement regulatory protein that counteracts complement-mediated inactivation of Newcastle Disease Virus
Rangaswamy, Udaya S. and Cotter, Christopher R. and Cheng, Xing and Jin, Hong and Chen, Zhongying,, 97, 1765-1770 (2016),
doi = https://doi.org/10.1099/jgv.0.000498,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Newcastle disease virus (NDV) is being developed as an oncolytic virus for virotherapy. In this study we analysed the regulation of complement-mediated inactivation of a recombinant NDV in different host cells. NDV grown in human cells was less sensitive to complement-mediated virus inactivation than NDV grown in embryonated chicken eggs. Additionally, NDV produced from HeLa-S3 cells is more resistant to complement than NDV from 293F cells, which correlated with higher expression and incorporation of complement regulatory proteins (CD46, CD55 and CD59) into virions from HeLa-S3 cells. Further analysis of the recombinant NDVs individually expressing the three CD molecules showed that CD55 is the most potent in counteracting complement-mediated virus inactivation. The results provide important information on selecting NDV manufacture substrate to mitigate complement-mediated virus inactivation.,
language=,
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