RT Journal Article SR Electronic(1) A1 Rangaswamy, Udaya S. A1 Cotter, Christopher R. A1 Cheng, Xing A1 Jin, Hong A1 Chen, ZhongyingYR 2016 T1 CD55 is a key complement regulatory protein that counteracts complement-mediated inactivation of Newcastle Disease Virus JF Journal of General Virology, VO 97 IS 8 SP 1765 OP 1770 DO https://doi.org/10.1099/jgv.0.000498 PB Microbiology Society, SN 1465-2099, AB Newcastle disease virus (NDV) is being developed as an oncolytic virus for virotherapy. In this study we analysed the regulation of complement-mediated inactivation of a recombinant NDV in different host cells. NDV grown in human cells was less sensitive to complement-mediated virus inactivation than NDV grown in embryonated chicken eggs. Additionally, NDV produced from HeLa-S3 cells is more resistant to complement than NDV from 293F cells, which correlated with higher expression and incorporation of complement regulatory proteins (CD46, CD55 and CD59) into virions from HeLa-S3 cells. Further analysis of the recombinant NDVs individually expressing the three CD molecules showed that CD55 is the most potent in counteracting complement-mediated virus inactivation. The results provide important information on selecting NDV manufacture substrate to mitigate complement-mediated virus inactivation., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000498