@article{mbs:/content/journal/jgv/10.1099/jgv.0.000707, author = "Hassan, Ahmed O and Vemula, Sai V and Sharma, Anurag and Bangari, Dinesh S and Mishra, Krishna K and Mittal, Suresh K", title = "155R is a novel structural protein of bovine adenovirus type 3, but it is not essential for virus replication", journal= "Journal of General Virology", year = "2017", volume = "98", number = "4", pages = "749-753", doi = "https://doi.org/10.1099/jgv.0.000707", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000707", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "bovine adenovirus type 3", keywords = "adenoviral novel structural protein", keywords = "155R protein", abstract = "Bovine adenovirus (AdV) type 3 (BAdV-3) E1 region shares functional homology with E1 of human AdV type C5. Sequence analysis of the BAdV-3 E1 region revealed the presence of a novel 155R ORF that is not observed in other AdVs, on the lower strand antiparallel to a portion of the E1B region. The 155R gene products in BAdV-3-infected cells were identified by Northern blot, reverse transcriptase PCR followed by sequencing and Western blot analysis using the155R-specific antibody. 155R seems to be a late protein and is present in purified BAdV-3 particles. Replication kinetics of BAdV mutants with either one (BAdV/155R/mt1) or two (BAdV/155R/mt2) stop codons in the 155R ORF were comparable to those of BAdV-3, indicating that 155R is not essential for virus replication in cell culture. These results suggest that 155R-deleted BAdV-3 vectors could be generated in a cell line that fully complements BAdV-3 E1 functions.", }