RT Journal Article SR Electronic(1) A1 Hassan, Ahmed O A1 Vemula, Sai V A1 Sharma, Anurag A1 Bangari, Dinesh S A1 Mishra, Krishna K A1 Mittal, Suresh KYR 2017 T1 155R is a novel structural protein of bovine adenovirus type 3, but it is not essential for virus replication JF Journal of General Virology, VO 98 IS 4 SP 749 OP 753 DO https://doi.org/10.1099/jgv.0.000707 PB Microbiology Society, SN 1465-2099, AB Bovine adenovirus (AdV) type 3 (BAdV-3) E1 region shares functional homology with E1 of human AdV type C5. Sequence analysis of the BAdV-3 E1 region revealed the presence of a novel 155R ORF that is not observed in other AdVs, on the lower strand antiparallel to a portion of the E1B region. The 155R gene products in BAdV-3-infected cells were identified by Northern blot, reverse transcriptase PCR followed by sequencing and Western blot analysis using the155R-specific antibody. 155R seems to be a late protein and is present in purified BAdV-3 particles. Replication kinetics of BAdV mutants with either one (BAdV/155R/mt1) or two (BAdV/155R/mt2) stop codons in the 155R ORF were comparable to those of BAdV-3, indicating that 155R is not essential for virus replication in cell culture. These results suggest that 155R-deleted BAdV-3 vectors could be generated in a cell line that fully complements BAdV-3 E1 functions., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000707