Dengue virus type 2 infects human endothelial cells through binding of the viral envelope glycoprotein to cell surface polypeptides
Wei, Hui-Yong and Jiang, Li-Fang and Fang, Dan-Yun and Guo, Hui-Yu,, 84, 3095-3098 (2003),
doi = https://doi.org/10.1099/vir.0.19308-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= The endothelial cell line ECV304, derived from human umbilical cord and identified to be susceptible to dengue virus type 2 (DEN-2) infection, was used to study the molecular mechanism of DEN-2 binding to endothelial cells. DEN-2 was found by virus overlay protein-binding assays (VOPBAs) to bind to three ECV304 cell membrane proteins with molecular masses of 29, 34 and 43 kDa. Only a single protein of 29 kDa was observed when VOPBAs were carried out using preparations of trypsin-treated ECV304 cells. Pre-incubation of live ECV304 cells in culture or cell membrane proteins in modified VOPBAs with the recombinant DEN-2 envelope glycoprotein (rEgp) inhibited DEN-2 infection and blocked virus binding to the three proteins identified. These results indicate that DEN-2 rEgp could bind to three proteins on the surface of ECV304 cells. This virus–cell interaction may be associated with the receptor complex specific for DEN-2 infection of endothelial cells.,
language=,
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