RT Journal Article SR Electronic(1) A1 Patel, Amrutlal K. A1 Tikoo, Suresh K.YR 2006 T1 293T cells expressing simian virus 40 T antigen are semi-permissive to bovine adenovirus type 3 infection JF Journal of General Virology, VO 87 IS 4 SP 817 OP 821 DO https://doi.org/10.1099/vir.0.81305-0 PB Microbiology Society, SN 1465-2099, AB Human cells do not normally support productive bovine adenovirus type 3 (BAdV-3) infection. Here, the outcome of BAdV-3 infection of both 293 cells and 293 cells modified to constitutively express the simian virus 40 (SV-40) T antigen (293T cells) was studied. Whereas BAdV-3 could efficiently infect 293 cells, there was a block in virus DNA replication, late-gene expression and virus production. In contrast, replication and efficient virus production could be detected in 293T cells infected with BAdV-3 or transfected with a replication-competent genomic BAdV-3 clone (pFBAV304). Early-phase gene expression was detected readily in both BAdV-3-infected 293 and 293T cells. However, the progression to efficient viral DNA synthesis and late-phase protein synthesis occurred only in 293T cells. Electron microscopy and virus growth kinetics demonstrated the formation of progeny virus in 293T cells. The SV-40 T antigens act to overcome a barrier in BAdV-3 DNA replication in 293 cells., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81305-0