@article{mbs:/content/journal/jgv/10.1099/vir.0.82867-0, author = "Onion, David and Crompton, Laura J. and Milligan, Donald W. and Moss, Paul A. H. and Lee, Steven P. and Mautner, Vivien", title = "The CD4+ T-cell response to adenovirus is focused against conserved residues within the hexon protein", journal= "Journal of General Virology", year = "2007", volume = "88", number = "9", pages = "2417-2425", doi = "https://doi.org/10.1099/vir.0.82867-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82867-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Adenovirus is a significant pathogen in immunocompromised patients and is widely utilized as a gene delivery vector, so a detailed understanding of the human immune response to adenovirus infection is critical. This study characterized the adenovirus-specific CD4+ T-cell response of healthy donors by incubation with whole virus or with individual hexon and fiber proteins. Adenovirus-specific CD4+ T cells averaged 0.26 % of the CD4+ T-cell pool and were detectable in all donors. T cells recognizing the highly conserved hexon protein accounted for 0.09 %, whereas no response was observed against the fiber protein. A panel of hexon-specific CD4+ T-cell clones was generated and shown to lyse targets infected with adenovirus from different serotypes and species. Three CD4 T-cell epitopes are described, which map to highly conserved regions of the hexon protein.", }