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Volume 66, Issue 1

is developing gradual increase in resistance to oral tetracyclines Free

Abstract

is an anaerobic bacterium that causes deep infection in organs and prosthetic joints, in addition to acne vulgaris. Many tetracycline-resistant strains have been isolated because oral tetracyclines are frequently used as an acne treatment against . In this study, we found a novel tetracycline resistance mechanism in . Three doxycycline-resistant (MIC: 16 µg ml) strains were isolated from 69 strains in acne patients in Japan between 2010 and 2011. Additionally, six insusceptible strains (MIC: 1–2 µg ml) that had reduced susceptibility compared to susceptible strains (MIC: ≤0.5 µg ml) were identified. All doxycycline-resistant strains had a G1036C mutation in the 16S rRNA gene in addition to an amino acid substitution in the ribosomal S10 protein encoded by . By contrast, insusceptible strains had an amino acid substitution in the S10 protein but no mutation in the 16S rRNA. When the mutant with decreased susceptibility to doxycycline was obtained , only the mutated S10 protein was found (MIC: 4 µg ml), not the mutated 16S rRNA gene. This result shows that the S10 protein amino acid substitution contributes to reduced doxycycline susceptibility in and suggests that tetracycline resistance is acquired through a 16S rRNA mutation after the S10 protein amino acid substitution causes reduced susceptibility.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA mutation , oral tetracycline resistance , Propionibacterium acnes , ribosomal protein S10 and rpsJ
© 2017 The Authors
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/content/journal/jmm/10.1099/jmm.0.000392
2017-01-01
2024-04-20
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References

  1. Mcdowell A, Gao A, Barnard E, Fink C, Murray PI et al. A novel multilocus sequence typing scheme for the opportunistic pathogen Propionibacterium acnes and characterization of type I cell surface-associated antigens. Microbiology 2011; 157:1990–2003 [View Article][PubMed]
     [Google Scholar]
  2. Ross JI, Snelling AM, Carnegie E, Coates P, Cunliffe WJ et al. Antibiotic-resistant acne: lessons from Europe. Br J Dermatol 2003; 148:467–478 [View Article][PubMed]
     [Google Scholar]
  3. Nakase K, Nakaminami H, Noguchi N, Nishijima S, Sasatsu M. First report of high levels of clindamycin-resistant Propionibacterium acnes carrying erm(X) in Japanese patients with acne vulgaris. J Dermatol 2012; 39:794–796 [View Article][PubMed]
     [Google Scholar]
  4. Coates P, Vyakrnam S, Eady EA, Jones CE, Cove JH et al. Prevalence of antibiotic-resistant propionibacteria on the skin of acne patients: 10-year surveillance data and snapshot distribution study. Br J Dermatol 2002; 146:840–848 [View Article][PubMed]
     [Google Scholar]
  5. Miyazaki E, Ando M, Fukami T, Nureki S, Eishi Y et al. Minocycline for the treatment of sarcoidosis: is the mechanism of action immunomodulating or antimicrobial effect?. Clin Rheumatol 2008; 27:1195–1197 [View Article][PubMed]
     [Google Scholar]
  6. Hu M, Nandi S, Davies C, Nicholas RA. High-level chromosomally mediated tetracycline resistance in Neisseria gonorrhoeae results from a point mutation in the rpsJ gene encoding ribosomal protein S10 in combination with the mtrR and penB resistance determinants. Antimicrob Agents Chemother 2005; 49:4327–4334 [View Article][PubMed]
     [Google Scholar]
  7. Lupien A, Gingras H, Leprohon P, Ouellette M. Induced tigecycline resistance in Streptococcus pneumoniae mutants reveals mutations in ribosomal proteins and rRNA. J Antimicrob Chemother 2015; 70:2973–2980 [View Article][PubMed]
     [Google Scholar]
  8. Ross JI, Eady EA, Cove JH, Cunliffe WJ. 16S rRNA mutation associated with tetracycline resistance in a gram-positive bacterium. Antimicrob Agents Chemother 1998; 42:1702–1705[PubMed]
     [Google Scholar]
  9. Nakase K, Nakaminami H, Takenaka Y, Hayashi N, Kawashima M et al. Relationship between the severity of acne vulgaris and antimicrobial resistance of bacteria isolated from acne lesions in a hospital in Japan. J Med Microbiol 2014; 63:721–728 [View Article][PubMed]
     [Google Scholar]
  10. Di Caprio R, Lembo S, Di Costanzo L, Balato A, Monfrecola G. Anti-inflammatory properties of low and high doxycycline doses: an in vitro study. Mediators Inflamm 2015; 2015:1–10 [View Article][PubMed]
     [Google Scholar]
  11. Ishida N, Nakaminami H, Noguchi N, Kurokawa I, Nishijima S et al. Antimicrobial susceptibilities of Propionibacterium acnes isolated from patients with acne vulgaris. Microbiol Immunol 2008; 52:621–624 [View Article][PubMed]
     [Google Scholar]
  12. CLSI Perfomance Standard for Antimicrobial Susceptibility Testing Approved standard M100-S21 Wayne, PA: Clinical and Laboratory Standards Institute; 2011
     [Google Scholar]
  13. CLSI National Committee for Clinical Laboratory Standards. Methods of Antimicrobial Susceptibility Testing of Anaerobic Bacteria, 8th ed. Approved Standard M11-A8 Wayne, PA: Clinical and Laboratory Standards Institute; 2012
     [Google Scholar]
  14. Spigaglia P, Barbanti F, Mastrantonio P. European Study Group on Clostridium difficile (ESGCD) Multidrug resistance in European Clostridium difficile clinical isolates. J Antimicrob Chemother 2011; 66:2227–2234 [View Article][PubMed]
     [Google Scholar]
  15. Scholz CF, Jensen A, Lomholt HB, Brüggemann H, Kilian M. A novel high-resolution single locus sequence typing scheme for mixed populations of Propionibacterium acnes in vivo. PLoS One 2014; 9:e104199 [View Article][PubMed]
     [Google Scholar]
  16. Iwaki M, Noguchi N, Nakaminami H, Sasatsu M, Ito M. [Antimicrobial activity and frequency of spontaneous gentamicin-resistant mutants in bacteria related skin infections]. Yakugaku Zasshi 2011; 131:1653–1659 (in Japanese) [View Article][PubMed]
     [Google Scholar]
  17. Bauer G, Berens C, Projan SJ, Hillen W. Comparison of tetracycline and tigecycline binding to ribosomes mapped by dimethylsulphate and drug-directed Fe2+ cleavage of 16S rRNA. J Antimicrob Chemother 2004; 53:592–599 [View Article][PubMed]
     [Google Scholar]
  18. Wimberly BT, Brodersen DE, Clemons WM, Morgan-Warren RJ, Carter AP et al. Structure of the 30S ribosomal subunit. Nature 2000; 407:327–339 [View Article][PubMed]
     [Google Scholar]
  19. Wei Y, Bechhofer DH. Tetracycline induces stabilization of mRNA in Bacillus subtilis. J Bacteriol 2002; 184:889–894 [View Article][PubMed]
     [Google Scholar]
  20. Cattoir V, Isnard C, Cosquer T, Odhiambo A, Bucquet F et al. Genomic analysis of reduced susceptibility to tigecycline in Enterococcus faecium. Antimicrob Agents Chemother 2015; 59:239–244 [View Article][PubMed]
     [Google Scholar]
  21. Fitz-Gibbon S, Tomida S, Chiu BH, Nguyen L, Du C et al. Propionibacterium acnes strain populations in the human skin microbiome associated with acne. J Invest Dermatol 2013; 133:2152–2160 [View Article][PubMed]
     [Google Scholar]
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Propionibacterium acnes is developing gradual increase in resistance to oral tetracyclines
J. Med. Microbiol. 66, 8 (2017); https://doi.org/10.1099/jmm.0.000392
/content/journal/jmm/10.1099/jmm.0.000392
/content/journal/jmm/10.1099/jmm.0.000392
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