1887

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Volume 62, Issue 9

Genetic characteristics of -positive plasmid in isolate separated from a clinical infectious patient Free

Abstract

This study reports an infectious case involving an NDM-1-producing and further explored the potential threat of the gene by analysing the characteristics of the NDM-1-encoding plasmid sequence. A -positive with high resistance to carbapenems was separated from a clinical patient suffering from a urinary tract infection. S1 nuclease-based plasmid analysis followed by Southern blot hybridization, a conjugation experiment and electrotransformation confirmed that the gene was located on a plasmid. High-throughput sequencing of the -postive plasmid (pCFNDM-CN) showed that it was a 54 kb IncX-type plasmid and contained a backbone region and a variable region with two β-lactamase genes ( and ). The NDM-1 composite transposon in the variable region was surrounded by IS and IS-truncated IS, and shared a high sequence similarity to the surrounding structure in spp. Our research suggested that the NDM-1 composite transposon might play an essential role in mobilization of the gene from spp. to Enterobacteriaceae.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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2013-09-01
2024-04-24
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Genetic characteristics of blaNDM-1-positive plasmid in Citrobacterfreundii isolate separated from a clinical infectious patient
J. Med. Microbiol. 62, 1332 (2013); https://doi.org/10.1099/jmm.0.057091-0
/content/journal/jmm/10.1099/jmm.0.057091-0
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