1887

Abstract

, a sulphide-tolerant, salt-tolerant member of the , was shown to produce molecular hydrogen on a mineral medium with lactate as electron donor and glutamate as nitrogen source. A maximum production of about 1·1 mmol H (mmol added lactate) was found at pH 6·75. The activity of hydrogenase and nitrogenase showed that hydrogenase activity (and thus the H recycling system) was greatest at pH 6·5 and 6·75 and thus, at least partially, was responsible for the lack of H production below 6·75, but that nitrogenase activity was greatest at between pH 6·5 and 7·0, and decreased to zero at pH 8·0, resulting in reduced H production at pH 7·5 and none at pH 8·0. When sufficient NH had accumulated in the culture, nitrogenase activity remained below the maximum value and no H production occurred.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-5-1243
1987-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/5/mic-133-5-1243.html?itemId=/content/journal/micro/10.1099/00221287-133-5-1243&mimeType=html&fmt=ahah

References

  1. Chaykin S. 1969; Assay of nicotinamide deaminase. Determination of ammonia by the indophenol reaction. Analytical Biochemistry 31:375–382
    [Google Scholar]
  2. Colbeau A., Chabert J., Vignais M. P. 1978; Hydrogenase activity in Rhodopseudomonas capsu- lata: stability and stabilization of the solubilized enzyme. In Hydrogenases: Their Catalytic Activity.. Structure and Function pp. 183–197 Schlegel H. G., Schneider R. Edited by Gottingen:: Goltze KG.;
    [Google Scholar]
  3. De vos P., Stevens P., De ley J. 1983; Hydrogen gas production from formate and glucose by different members of the Enterobacteriaceae. Biotechnology Letters 5:69–74
    [Google Scholar]
  4. Gest H., Kamen M. P. 1949; Photoproduction of molecular hydrogen by Rhodospirillum rubrum. Science 109:558–559
    [Google Scholar]
  5. Gillis M., Dejonghe J., Smet A., Onghenae G., De ley J. 1982; Intra- and intergeneric similarities of the ribosomal ribonucleic acid cistrons in the Rhodospirillaceae. Abstract (A16) of the IVth International Symposium on Photosynthetic Prokaryotes, Bombannes, France
    [Google Scholar]
  6. Hansen T. A., Veldkamp H. 1973; Rhodopseudomonas sulfidophila nov. spec., a new species of the purple nonsulfur bacteria. Archives of Microbiology 92:45–56
    [Google Scholar]
  7. Kelley B. C., Jouanneau Y., Vignais P. M. 1979; Nitrogenase activity in Rhodopseudomonas sulfidophila. Archives of Microbiology 122:145–152
    [Google Scholar]
  8. Madigan M., Cox S. S., Stegeman R. A. 1984; Nitrogen fixation and nitrogenase activities in members of the family Rhodospirillaceae. Journal of Bacteriology 157:73–78
    [Google Scholar]
  9. Meyer B., Kelley B. C., Vignais P. M. 1978; Nitrogen fixation and hydrogen metabolism in photosynthetic bacteria. Biochimie 60:245–260
    [Google Scholar]
  10. Stevens P., Van der sypt H., De vos P., De ley J. 1983; Comparative study on H2 evolution from DL-lactate, acetate and butyrate by different strains of Rhodopseudomonas capsulata in a new type of reactor. Biotechnology Letters 5:369–374
    [Google Scholar]
  11. Stevens P., Plovie N, De ley J. 1986; Photoproduction of molecular hydrogen by Rhodobacter sulfidophilus.. Systematic and Applied Microbiology 8:19–23
    [Google Scholar]
  12. Vignais P. M., Colbeau A., Willison J. C., Jouanneau Y. 1985; Hydrogenase, nitrogenase, and hydrogen metabolism in the photosynthetic bacteria. Advances in Microbial Physiology 26:155–234
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-5-1243
Loading
/content/journal/micro/10.1099/00221287-133-5-1243
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error