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Volume 143, Issue 11

Sequence analysis and characterization of øO1205, a temperate bacteriophage infecting CNRZ1205 Free

Abstract

The complete nucleotide sequence of øO1205, a temperate bacteriophage infecting strain CNRZ1205, was determined. The phage genome has a unit length of 43075 bp and appears to be packaged by the so-called headful mechanism. The genomic organization and structure of øO1205 resemble those of several temperate lactococcal phages that display a life-cycle-specific organization, where ORFs believed to be involved in the lysogenic life-cycle are clustered and arranged in an orientation opposite to the ORFs supposedly involved in the lytic life-cycle. Database searches revealed putative functions for several identified ORFs and further indicated that øO1205 is genetically related to a particular group of lactococcal phages. Three genes encoding the major structural proteins were identified on the øO1205 genome. The phage attachment site the bacterial attachment site and the two phage/chromosome junctions and attR were identified and found to contain a 40 bp common core sequence.

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): attachment site , bacteriophage , sequence analysis , Streptococcus thermophilus, and structural proteins
© Society for General Microbiology 1997
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1997-11-01
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Sequence analysis and characterization of øO1205, a temperate bacteriophage infecting Streptococcus thermophilus CNRZ1205
Microbiology 143, 3417 (1997); https://doi.org/10.1099/00221287-143-11-3417
/content/journal/micro/10.1099/00221287-143-11-3417
/content/journal/micro/10.1099/00221287-143-11-3417
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