RT Journal Article SR Electronic(1) A1 Zhang, Lihong A1 Jacobsson, Karin A1 Vasi, Jozsef A1 Lindberg, Martin A1 Frykberg, LarsYR 1998 T1 A second IgG-binding protein in Staphylococcus aureus JF Microbiology, VO 144 IS 4 SP 985 OP 991 DO https://doi.org/10.1099/00221287-144-4-985 PB Microbiology Society, SN 1465-2080, AB Most strains of Staphylococcus aureus express IgG-binding activity and this binding has been considered to be solely mediated by protein A. However, the existence of a second gene in S. aureus strain 8325-4 encoding an IgG-binding polypeptide was recently reported. This novel IgG-binding polypeptide was found after panning a shotgun phage display library, made from chromosomal DNA, against immobilized human IgG. The complete gene (sbi) encoding this novel IgG-binding protein, denoted protein Sbi, has now been cloned and sequenced. Analysis of other S. aureus strains showed that this gene is not unique for strain 8325-4. The protein consists of 436 amino acids and exhibits an immunoglobulin-binding specificity similar to protein A. Furthermore, it is shown that Sbi is highly expressed in strain Newman 4, which shows that IgG-binding activity in S. aureus can be mediated by proteins other than protein A., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-144-4-985