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Volume 147, Issue 10

Genetic diversity of fowl cholera isolates as demonstrated by ribotyping and 16S rRNA and partial sequence comparisons

The GenBank accession numbers for the 16S rRNA sequences of strain HIM 830-7 (NCTC 10204) and 77179 of subsp. and HIM 746-6 (NCTC 11619) of subsp. are AF326323, AF326324 and AF326325, respectively; those for the nucleotide sequences are in Fig. 3.

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Abstract

The genetic diversity of , the aetiological agent of fowl cholera, was investigated. The strain collection comprised 69 clinical isolates representing a wide spectrum of hosts and geographic origin. The three type strains for the subspecies of were also included. Avian isolates of subsp. and subsp. did not represent separate lines by II ribotyping and the two type strains of mammalian origin (porcine and cat bite) seemed to be representative of avian strains of subspp. and . By ribotyping, all subsp. strains, except one chicken isolate and the type strain, clustered together. This indicated that the bovine type strain was not representative of this subspecies and that most strains of subsp. are genetically related and may be distantly related to other isolates, including those of avian origin. By 16S rRNA and sequence comparisons of selected strains, including both isolated from birds and mammals and selected distantly related species associated with birds and mammals, it was found that is monophyletic. Extended DNA–DNA hybridizations are highly indicated since strains may exist which would connect the existing subspecies at species level. The considerable genetic diversity of fowl cholera isolates is probably related to the clonal nature of this organism, resulting in many divergent lines.

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Keyword(s): aetiology , ATP synthase , clonal lines , evolution , MLEE, multilocus enzyme electrophoresis and phylogeny
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2001-10-01
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Genetic diversity of Pasteurella multocida fowl cholera isolates as demonstrated by ribotyping and 16S rRNA and partial atpD sequence comparisonsThe GenBank accession numbers for the 16S rRNA sequences of strain HIM 830-7T (NCTC 10204T) and 77179 of P. multocida subsp. gallicida and HIM 746-6T (NCTC 11619T) of P. multocida subsp. septica are AF326323, AF326324 and AF326325, respectively; those for the atpD nucleotide sequences are in Fig. 3.
Microbiology 147, 2739 (2001); https://doi.org/10.1099/00221287-147-10-2739
/content/journal/micro/10.1099/00221287-147-10-2739
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