%0 Journal Article %A Mullins, Paul H. %A Gürtler, Hanne %A Wellington, Elizabeth M. H. %T Selective recovery of Streptosporangium fragile from soil by indirect immunomagnetic capture %D 1995 %J Microbiology, %V 141 %N 9 %P 2149-2156 %@ 1465-2080 %R https://doi.org/10.1099/13500872-141-9-2149 %K immunocapture %K soil %K selective isolation %K magnetic beads %K Streptosporangium %I Microbiology Society, %X A polyclonal antibody raised to Streptosporangium fragile spores reacted strongly and specifically with the immunizing strain and to a number of related species of Streptosporangium, as determined by dot immunoblotting. An indirect immunomagnetic capture method was developed for the recovery of the target organism from sterile and non-sterile soil, using sheep anti-rabbit M-280 Dynabeads. The effects of different soil blocking agents, antibody labelling concentrations and spore/Dynabead capture times on the recovery of S. fragile spores were investigated. Pre-blocking of antibody binding sites within the soil, with either 2% partially hydrolysed gelatin or 10% skimmed milk, was essential prior to immunomagnetic capture. Increasing the capture time from 15 to 60 min did not affect spore recovery; however, a 10-fold decline in the magnetic bead concentration did result in a significantly lower recovery of spores from soil. S. fragile was selectively enriched (1:190-fold) when present as a mixed population with Arthrobacter oxydans in sterile soil. The indirect immunomagnetic capture method was used to selectively recover S. fragile spores seeded into non-sterile soil, although some background binding of non-target bacteria was noted. The target was successfully recovered from a sterile soil microcosm after 14 d incubation and the capture rate was increased by the inclusion of an initial soil dispersion and biomass concentration procedure, using the ion-exchange resin Chelex 100. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-141-9-2149