@article{mbs:/content/journal/micro/10.1099/mic.0.000310, author = "Parmeciano Di Noto, Gisela and Jara, Eugenio and Iriarte, Andrés and Centrón, Daniela and Quiroga, Cecilia", title = "Genome analysis of a clinical isolate of Shewanella sp. uncovered an active hybrid integrative and conjugative element carrying an integron platform inserted in a novel genomic locus", journal= "Microbiology", year = "2016", volume = "162", number = "8", pages = "1335-1345", doi = "https://doi.org/10.1099/mic.0.000310", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000310", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "Shewanella", keywords = "integrative conjugative element", keywords = "integrase", keywords = "clinical isolate", keywords = "antimicrobial resistance", keywords = "horizontal transfer event", abstract = " Shewanella spp. are currently considered to be emerging pathogens that can code for a bla OXA carbapenemase in their chromosome. Complete genome analysis of the clinical isolate Shewanella sp. Sh95 revealed that this strain is a novel species, which shares a lineage with marine isolates. Characterization of its resistome showed that it codes for genes drfA15, qacH and bla OXA-48. We propose that Shewanella sp. Sh95 acts as reservoir of bla OXA-48. Moreover, analysis of mobilome showed that it contains a novel integrative and conjugative element (ICE), named ICESh95. Comparative analysis between the close relatives ICESpuPO1 from Shewanella sp. W3-18-1 and ICE SXTMO10 from Vibrio cholerae showed that ICESh95 encompassed two new regions, a type III restriction modification system and a multidrug resistance integron. The integron platform contained a novel arrangement formed by gene cassettes drfA15 and qacH, and a class C-attC group II intron. Furthermore, insertion of ICESh95 occurred at a unique target site, which correlated with the presence of a different xis/int module. Mobility of ICESh95 was assessed and demonstrated its ability to self-transfer with high efficiency to different species of bacteria. Our results show that ICESh95 is a self-transmissible, mobile element, which can contribute to the dissemination of antimicrobial resistance; this is clearly a threat when natural bacteria from water ecosystems, such as Shewanella, act as vectors in its propagation.", }