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Volume 155, Issue 2

The Hog1 MAP kinase controls respiratory metabolism in the fungal pathogen Free

Abstract

Signal transduction pathways mediated by mitogen-activated protein kinases (MAPKs) play crucial roles in eukaryotic cells. In the pathogenic fungus the HOG MAPK pathway regulates the response to external stresses (osmotic and oxidative among others) and is involved in morphogenesis and virulence. We show here that the lack of the Hog1 MAPK increases the sensitivity of this fungus to inhibitors of the respiratory chain. mutants also show an enhanced basal respiratory rate compared to parental strains, and higher levels of intracellular reactive oxygen species despite an increased expression of detoxifying enzymes. We also demonstrate that although oxidative phosphorylation is essentially unaffected, mutants have an altered mitochondrial membrane potential. Data indicate that -defective mutants are more dependent on mitochondrial ATP synthesis, probably due to an increased cellular ATP demand. Our results therefore link a MAPK pathway with respiratory metabolism in pathogenic fungi.

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Keyword(s): AOX, alternative oxidase , carboxy-H2DCFDA, carboxy-2′7′-dichlorodihydrofluorescein , DNP, 2,4-dinitrophenol , FCCP, carbonylcyanide p-trifluoromethoxyphenylhydrazone , HEt, dihydroethidium , MAPK, mitogen-activated protein kinase , MCLA, 2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazol[1,2-α] pyrazin-3-one, hydrochloride , PQ, paraquat , Rh123, rhodamine 123 , ROS, reactive oxygen species and SHAM, salicylhydroxamic acid
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2009-02-01
2024-04-25
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The Hog1 MAP kinase controls respiratory metabolism in the fungal pathogen Candida albicans
Microbiology 155, 413 (2009); https://doi.org/10.1099/mic.0.023309-0
/content/journal/micro/10.1099/mic.0.023309-0
/content/journal/micro/10.1099/mic.0.023309-0
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