RT Journal Article SR Electronic(1) A1 Tahrioui, Ali A1 Quesada, Emilia A1 Llamas, InmaculadaYR 2011 T1 The hanR/hanI quorum-sensing system of Halomonas anticariensis, a moderately halophilic bacterium JF Microbiology, VO 157 IS 12 SP 3378 OP 3387 DO https://doi.org/10.1099/mic.0.052167-0 PB Microbiology Society, SN 1465-2080, AB Quorum sensing is a cell density-dependent gene expression mechanism found in many Gram-negative bacteria which involves the production of signal molecules such as N-acylhomoserine lactones (AHLs). One significant group of micro-organisms in which quorum sensing has not been previously studied, however, are the moderate halophiles. We describe here the results of our studies of the quorum-sensing system in Halomonas anticariensis FP35T, which is composed of luxR/luxI homologues: hanR (the putative transcriptional regulator gene) and hanI (the autoinducer synthase gene). To understand how the hanR/hanI system is organized and regulated we conducted RT-PCR and quantitative real-time PCR assays. Transcriptional analysis indicated that the hanR and hanI genes are on the same transcript and that their transcription is growth phase-dependent. HanI seems to be the only autoinducer synthase responsible for the synthesis of AHLs by the bacterium, since the inactivation of hanI resulted in the complete loss of its AHLs. We also found that the hanI gene appears to be transcribed from its own promoter and that its expression does not depend upon HanR. This finding was supported by the fact that the FP35hanR mutant showed AHL-producing activity and hanI expression similar to that of the wild-type strain, the latter being measured by RT-PCR. Moreover, hanR is expressed from its own promoter and appears to be independent of the AHL signalling molecules produced by HanI., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.052167-0