1887

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Volume 160, Issue 9

Characterization of stipe elongation of the mushroom Free

Abstract

Previously, we observed an acid-induced short-term wall extension in apical stipes during a 15 min period after a change from a neutral to an acidic pH. This acid-induced stipe wall extension was eliminated by heating and reconstituted by a snail expansin-like protein, although we failed to isolate any endogenous expansin-like protein from because of its limited 1 mm fast elongation region. In this study, we report that stipes possess a 9 mm fast elongation apical region, which is suitable as a model material for wall extension studies. The elongating apical stipe showed two phases of acid-induced wall extension, an initial quick short-term wall extension during the first 15 min and a slower, gradually decaying long-term wall extension over the subsequent 2 h. After heating or protein inactivation pretreatment, apical stipes lost the long-term wall extension, retaining a slower short-term wall extension, which was reconstituted by an expansin-like snail protein. In contrast, the non-elongating basal stipes showed only a weaker short-term wall extension. We propose that the long-term wall extension is a protein-mediated process involved in stipe elongation, whereas the short-term wall extension is a non-protein mediated process not involved in stipe elongation.

  • Received:
  • Accepted:
  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31170028)
  • Priority Academic Development Program of Jiangsu Higher Education Institutions and the Scientific Innovation Research Program of Graduates at Nanjing Normal University (Award CXLX13-384)
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2014-09-01
2024-04-19
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Characterization of stipe elongation of the mushroom Coprinopsis cinerea
Microbiology 160, 1893 (2014); https://doi.org/10.1099/mic.0.079418-0
/content/journal/micro/10.1099/mic.0.079418-0
/content/journal/micro/10.1099/mic.0.079418-0
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