%0 Journal Article %A Zhang, Wenming %A Wu, Xiuxiu %A Zhou, Yajun %A Liu, Zhonghua %A Zhang, Wen %A Niu, Xin %A Zhao, Yan %A Pei, Siyu %A Zhao, Yang %A Yuan, Sheng %T Characterization of stipe elongation of the mushroom Coprinopsis cinerea %D 2014 %J Microbiology, %V 160 %N 9 %P 1893-1902 %@ 1465-2080 %R https://doi.org/10.1099/mic.0.079418-0 %I Microbiology Society, %X Previously, we observed an acid-induced short-term wall extension in Flammulina velutipes apical stipes during a 15 min period after a change from a neutral to an acidic pH. This acid-induced stipe wall extension was eliminated by heating and reconstituted by a snail expansin-like protein, although we failed to isolate any endogenous expansin-like protein from F. velutipes because of its limited 1 mm fast elongation region. In this study, we report that Coprinopsis cinerea stipes possess a 9 mm fast elongation apical region, which is suitable as a model material for wall extension studies. The elongating apical stipe showed two phases of acid-induced wall extension, an initial quick short-term wall extension during the first 15 min and a slower, gradually decaying long-term wall extension over the subsequent 2 h. After heating or protein inactivation pretreatment, apical stipes lost the long-term wall extension, retaining a slower short-term wall extension, which was reconstituted by an expansin-like snail protein. In contrast, the non-elongating basal stipes showed only a weaker short-term wall extension. We propose that the long-term wall extension is a protein-mediated process involved in stipe elongation, whereas the short-term wall extension is a non-protein mediated process not involved in stipe elongation. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.079418-0