Characterization of stipe elongation of the mushroom Coprinopsis cinerea
Zhang, Wenming and Wu, Xiuxiu and Zhou, Yajun and Liu, Zhonghua and Zhang, Wen and Niu, Xin and Zhao, Yan and Pei, Siyu and Zhao, Yang and Yuan, Sheng,, 160, 1893-1902 (2014),
doi = https://doi.org/10.1099/mic.0.079418-0,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Previously, we observed an acid-induced short-term wall extension in Flammulina velutipes apical stipes during a 15 min period after a change from a neutral to an acidic pH. This acid-induced stipe wall extension was eliminated by heating and reconstituted by a snail expansin-like protein, although we failed to isolate any endogenous expansin-like protein from F. velutipes because of its limited 1 mm fast elongation region. In this study, we report that Coprinopsis cinerea stipes possess a 9 mm fast elongation apical region, which is suitable as a model material for wall extension studies. The elongating apical stipe showed two phases of acid-induced wall extension, an initial quick short-term wall extension during the first 15 min and a slower, gradually decaying long-term wall extension over the subsequent 2 h. After heating or protein inactivation pretreatment, apical stipes lost the long-term wall extension, retaining a slower short-term wall extension, which was reconstituted by an expansin-like snail protein. In contrast, the non-elongating basal stipes showed only a weaker short-term wall extension. We propose that the long-term wall extension is a protein-mediated process involved in stipe elongation, whereas the short-term wall extension is a non-protein mediated process not involved in stipe elongation.,
language=,
type=