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Volume 67, Issue 2

gen. nov., sp. nov., a sugar-degrading member of the isolated from ant guts Free

Abstract

‘turtle’ ants host a core group of gut–associated symbionts, but their potential contributions to ant nutrition and disease resistance remain uncharacterized . To gain a better understanding of the metabolic capability of core symbionts belonging to the , we cultivated and characterized strain CAG32 from the guts of ants. Strain CAG32 was rod-shaped, Gram-stain-negative, motile and formed pale-white colonies on trypticase soy agar. Optimum growth occurred under an atmosphere of 20 % O supplemented with 1 % CO. Strain CAG32 grew under NaCl concentrations of 0–2.0 %, temperatures of 23–47 °C and pH values of 4.0–8.0, and was capable of producing -butyric acid and degrading carbohydrates for growth. The G+C content of the genomic DNA was 59.2±0.6 mol% and the major fatty acids were C, C 7/C 6, C cylcopropane, C and C 3-OH/C iso I. The only respiratory quinone detected was ubiquinone-8 (Q-8) and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on phylogenetic analysis of the 16S rRNA gene sequence, strain CAG32 shared 96.9 % nucleotide similarity with its closest cultivated neighbours Se-1111R and ATCC 19395. This, combined with differences in the phenotypic and biochemical profile from neighbouring strains, warrants the classification of strain CAG32 as representing a novel species of a new genus within the family . The name gen. nov., sp. nov. is proposed. The type strain of is CAG32 (=NCIMB 15010=DSM 100909).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Burkholderiales , Cephalotes rohweri , gut microbiota , Saccharedens versatilis , short-chain fatty acid and symbiont
© 2017 IUMS
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2017-02-01
2024-05-09
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Saccharedens versatilis gen. nov., sp. nov., a sugar-degrading member of the Burkholderiales isolated from Cephalotes rohweri ant guts
Int J Syst Evol Microbiol 67, 447 (2017); https://doi.org/10.1099/ijsem.0.001650
/content/journal/ijsem/10.1099/ijsem.0.001650
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