1887

Abstract

The mechanism involved in the action of eosin-methylene blue agar (EMB) in differentiating from was investigated using prototrophic strains of these organisms and a Lac auxotroph, X-961. The final pH of the EMB agar resulting from growth of these organisms at the expense of lactose was shown to play a critical role in the differential action. An EMB complex, which formed under acidic conditions, appeared to be involved in the differential action of this medium. The molecular weight of the complex indicated an eosin to methylene blue ratio of 1 to 1. Ultraviolet and infrared spectral data indicated the occurrence of an amide bond between the dyes when complexing occurred. On this basis, a mechanism for complexing of eosin and methylene blue, under acidic conditions, was proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-24-2-221
1974-04-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/24/2/ijs-24-2-221.html?itemId=/content/journal/ijsem/10.1099/00207713-24-2-221&mimeType=html&fmt=ahah

References

  1. Colthup N.B., Daly L.H., Wiberley S.E. 1964; Introduction to infrared and raman spectros copy p.263-265,332, 382-384. Academic Press Inc New York:
    [Google Scholar]
  2. Holt-Harris J.E., Teague O. 1916; A new culture medium for the isolation of Bacillus typhosus from stools. J. Infect. Dis 18:596–600
    [Google Scholar]
  3. Levine M. 1918; Differentiation of B. coli and B.aerogenes on a simplified eosin-methylene blue agar. J. Infect. Dis 23:43–47
    [Google Scholar]
  4. Rao C.N.R. 1967; Ultraviolet and visible spectroscopy, chemical applications. p. 193 Plenum Press; New York:
    [Google Scholar]
  5. Schwarz J.C.P. 1964; Physical methods in organic chemistry. p. 250–252 Holden-Day,Inc; SanFrancisco:
    [Google Scholar]
  6. Wilson T.M. 1907; Chemical and staining properties of derivatives of methylene blue and eosin. J.Exp. Med 9:645–670
    [Google Scholar]
  7. Wynne E.S., Rode L.J., Hayward A. E. 1942; Mechanism of the selective action of eosin-methylene blue agar on the enteric group. Stain Technol 17:11–20
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-24-2-221
Loading
/content/journal/ijsem/10.1099/00207713-24-2-221
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error