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f gen. nov., sp. nov., a human intestinal mucin-degrading bacterium

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  • The diversity of mucin-degrading bacteria in the human intestine was investigated by combining culture and 16S rRNA-dependent approaches. A dominant bacterium, strain Muc, was isolated by dilution to extinction of faeces in anaerobic medium containing gastric mucin as the sole carbon and nitrogen source. A pure culture was obtained using the anaerobic soft agar technique. Strain Muc was a Gram-negative, strictly anaerobic, non-motile, non-spore-forming, oval-shaped bacterium that could grow singly and in pairs. When grown on mucin medium, cells produced a capsule and were found to aggregate. Strain Muc could grow on a limited number of sugars, including -acetylglucosamine, -acetylgalactosamine and glucose, but only when a protein source was provided and with a lower growth rate and final density than on mucin. The G+C content of DNA from strain Muc was 47·6 mol%. 16S rRNA gene sequence analysis revealed that the isolate was part of the division . The closest described relative of strain Muc was (92 % sequence similarity). Remarkably, the 16S rRNA gene sequence of strain Muc showed 99 % similarity to three uncultured colonic bacteria. According to the data obtained in this work, strain Muc represents a novel bacterium belonging to a new genus in subdivision 1 of the ; the name gen. nov., sp. nov. is proposed; the type strain is Muc (=ATCC BAA-835=CIP 107961).

  • Published online ahead of print on 14 May 2004 as DOI 10.1099/ijs.0.02873-0.

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Muc is AY271254.

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  • Chin, K.-J., Liesack, W. & Janssen, P. H. ( 2001; ). Opitutus terrae gen. nov., sp. nov., to accommodate novel strains of the division ‘Verrucomicrobia’ isolated from rice paddy soil. Int J Syst Evol Microbiol 51, 1965–1968.[CrossRef]
    [Google Scholar]
  • Duncan, S. H., Hold, G. L., Barcenilla, A., Stewart, C. S. & Flint, H. J. ( 2002; ). Roseburia intestinalis sp. nov., a novel saccharolytic, butyrate-producing bacterium from human faeces. Int J Syst Evol Microbiol 52, 1615–1620.[CrossRef]
    [Google Scholar]
  • Hedlund, B. P., Gosink, J. J. & Staley, J. T. ( 1997; ). Verrucomicrobia div. nov., a new division of the Bacteria containing three new species of Prosthecobacter. Antonie van Leeuwenhoek 72, 29–38.[CrossRef]
    [Google Scholar]
  • Hold, G. L., Pryde, S. E., Russell, V. J., Furrie, E. & Flint, H. J. ( 2002; ). Assessment of microbial diversity in human colonic samples by 16S rDNA sequence analysis. FEMS Microbiol Ecol 39, 33–39.[CrossRef]
    [Google Scholar]
  • Hooper, L. V. & Gordon, J. I. ( 2001; ). Commensal host-bacterial relationships in the gut. Science 292, 1115–1118.[CrossRef]
    [Google Scholar]
  • Hoskins, L. C. & Boulding, E. T. ( 1981; ). Mucin degradation in human colon ecosytems. Evidence for the existence and role of bacterial subpopulations producing glycosidases as extracellular enzymes. J Clin Invest 67, 163–172.[CrossRef]
    [Google Scholar]
  • Hugenholtz, P., Goebel, B. M. & Pace, N. R. ( 1998; ). Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. J Bacteriol 180, 4765–4774.
     [Google Scholar]
  • Janssen, P. H., Schuhmann, A., Mörschel, E. & Rainey, F. A. ( 1997; ). Novel anaerobic ultramicrobacteria belonging to the Verrucomicrobiales lineage of bacterial descent isolated by dilution culture from anoxic rice paddy soil. Appl Environ Microbiol 63, 1382–1388.
     [Google Scholar]
  • Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: John Wiley.
  • Lawson, A. J., On, S. L. W., Logan, J. M. J. & Stanley, J. ( 2001; ). Campylobacter hominis sp. nov., from the human gastrointestinal tract. Int J Syst Evol Microbiol 51, 651–660.
     [Google Scholar]
  • Lesuffleur, T., Barbat, A., Dussaulx, E. & Zweibaum, A. ( 1990; ). Growth adaptation to methotrexate of HT-29 human colon carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells. Cancer Res 50, 6334–6343.
     [Google Scholar]
  • Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  • Miller, R. S. & Hoskins, L. C. ( 1981; ). Mucin degradation in human colon ecosystems. Fecal population densities of mucin-degrading bacteria estimated by a ‘most probable number’ method. Gastroenterology 81, 759–765.
     [Google Scholar]
  • Muyzer, G., de Waal, E. C. & Uitterlinden, A. G. ( 1993; ). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59, 695–700.
     [Google Scholar]
  • Nübel, U., Engelen, B., Felske, A., Snaidr, J., Wieshuber, A., Amann, R. I., Ludwig, W. & Backhaus, H. ( 1996; ). Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J Bacteriol 178, 5636–5643.
     [Google Scholar]
  • Plugge, C. M., Zoetendal, E. G. & Stams, A. J. M. ( 2000; ). Caloramator coolhaasii sp. nov., a glutamate-degrading, moderately thermophilic anaerobe. Int J Syst Evol Microbiol 50, 1155–1162.[CrossRef]
    [Google Scholar]
  • Ruseler-van Embden, J. G. H., van Lieshout, L. M. C., Gosselink, M. J. & Marteau, P. ( 1995; ). Inability of Lactobacillus casei strain GG, L. acidophilus, and Bifidobacterium bifidum to degrade intestinal mucus glycoproteins. Scand J Gastroenterol 30, 675–680.[CrossRef]
    [Google Scholar]
  • Salyers, A. A., West, S. E. H., Vercellotti, J. R. & Wilkins, T. D. ( 1977; ). Fermentation of mucins and plant polysaccharides by anaerobic bacteria from the human colon. Appl Environ Microbiol 34, 529–533.
     [Google Scholar]
  • Salzman, N. H., de Jong, H., Paterson, Y., Harmsen, H. J. M., Welling, G. W. & Bos, N. A. ( 2002; ). Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria. Microbiology 148, 3651–3660.
     [Google Scholar]
  • Sanguinetti, C. J., Dias Neto, E. & Simpson, A. J. G. ( 1994; ). Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 17, 914–921.
     [Google Scholar]
  • Schlesner, H. ( 1987; ). Verrucomicrobium spinosum gen. nov., sp. nov. a fimbriated prosthecate bacterium. Syst Appl Microbiol 10, 54–56.[CrossRef]
    [Google Scholar]
  • Schwiertz, A., Hold, G. L., Duncan, S. H., Gruhl, B., Collins, M. D., Lawson, P. A., Flint, H. J. & Blaut, M. ( 2002; ). Anaerostipes caccae gen. nov., sp. nov., a new saccharolytic, acetate-utilising, butyrate-producing bacterium from human faeces. Syst Appl Microbiol 25, 46–51.[CrossRef]
    [Google Scholar]
  • Simmering, R., Taras, D., Schwiertz, A., Le Blay, G., Gruhl, B., Lawson, P. A., Collins, M. D. & Blaut, M. ( 2002; ). Ruminococcus luti sp. nov., isolated from a human faecal sample. Syst Appl Microbiol 25, 189–193.[CrossRef]
    [Google Scholar]
  • Staley, J. T., Bont, J. A. M. & Jonge, K. ( 1976; ). Prosthecobacter fusiformis nov. gen. et sp., the fusiform caulobacter. Antonie van Leeuwenhoek 42, 333–342.[CrossRef]
    [Google Scholar]
  • Stams, A. J. M., van Dijk, J. B., Dijkema, C. & Plugge, C. M. ( 1993; ). Growth of syntrophic propionate-oxidizing bacteria with fumarate in the absence of methanogenic bacteria. Appl Environ Microbiol 59, 1114–1119.
     [Google Scholar]
  • Strunk, O. & Ludwig, W. ( 1995; ). arb – a software environment for sequence data. Department of Microbiology, Technical University of Munich, Munich, Germany (http://www.arb-home.de/).
  • Suau, A., Bonnet, R., Sutren, M., Godon, J.-J., Gibson, G. R., Collins, M. D. & Doré, J. ( 1999; ). Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 65, 4799–4807.
     [Google Scholar]
  • Taras, D., Simmering, R., Collins, M. D., Lawson, P. A. & Blaut, M. ( 2002; ). Reclassification of Eubacterium formicigenerans Holdeman and Moore 1974 as Dorea formicigenerans gen. nov., comb. nov., and description of Dorea longicatena sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 52, 423–428.
     [Google Scholar]
  • Vaughan, E. E., Schut, F., Heilig, H. G. H. J., Zoetendal, E. G., de Vos, W. M. & Akkermans, A. D. L. ( 2000; ). A molecular view of the intestinal ecosystem. Curr Issues Intest Microbiol 1, 1–12.
     [Google Scholar]
  • Willis, C. L., Cummings, J. H., Neale, G. & Gibson, G. R. ( 1996; ). In vitro effects of mucin fermentation on the growth of human colonic sulphate-reducing bacteria. Anaerobe 2, 117–122.[CrossRef]
    [Google Scholar]
  • Wilson, K. H. & Blitchington, R. B. ( 1996; ). Human colonic biota studied by ribosomal DNA sequence analysis. Appl Environ Microbiol 62, 2273–2278.
     [Google Scholar]
  • Zhou, J. S., Gopal, P. K. & Gill, H. S. ( 2001; ). Potential probiotic lactic acid bacteria Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019) do not degrade gastric mucin in vitro. Int J Food Microbiol 63, 81–90.[CrossRef]
    [Google Scholar]
  • Zoetendal, E. G., Akkermans, A. D. L. & de Vos, W. M. ( 1998; ). Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl Environ Microbiol 64, 3854–3859.
     [Google Scholar]
  • Zoetendal, E. G., Plugge, C. M., Akkermans, A. D. L. & de Vos, W. M. ( 2003; ). Victivallis vadensis gen. nov., sp. nov., a sugar-fermenting anaerobe from human faeces. Int J Syst Evol Microbiol 53, 211–215.[CrossRef]
    [Google Scholar]
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2004-09-01
2019-05-22
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