1887

Abstract

Abstract

The deoxyribonucleic acids (DNA) of ATCC 10953 and ATCC 23726 were submitted to DNA-DNA hybridization procedures. A polynucleotide homology of 78% was demonstrated, indicating that the recent grouping of all oral strains into one species, should be reevaluated. The polynucleotide homology between ATCC 19616 and the two strains of tested was less than 30%. The guanine plus cytosine (G+C) values of the DNA and strains tested were similar and unusually low (24.5 to 25.6 mol%). Since bacteria of the family reportedly have G+C values of 41 to 42 mol%, the placing of the genera and into this family is questionable. The similar unusually low G+C values of the DNA of and may indicate a close phylogenetic relationship for these genera.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-26-3-301
1976-07-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/26/3/ijs-26-3-301.html?itemId=/content/journal/ijsem/10.1099/00207713-26-3-301&mimeType=html&fmt=ahah

References

  1. Boe J. 1941; Fusobacterium. Studies on its bacteriology, serology and pathogenicity. Nor. Vidensk.- Akad. Oslo. I. Mat.-Naturv. Klasse 9 1 191
    [Google Scholar]
  2. Brenner D. J., Fanning G. R., Steigerwalt A. G. 1974; Polynucleotide sequence relatedness in Edwardsiella tarda.. Int. J. Syst, Bacteriol 24 186 190
    [Google Scholar]
  3. Brenner D. J., Fanning G. R., Steigerwalt A. G. 1974; Deoxyribonucleic acid relatedness among er-winiae and other Enterobacteriaceae: the gall, wilt, and dry-necrosis organisms (genus Erwinia Winslow et al., sensu stricto).. Int. J. Syst. Bacteriol 24 197 204
    [Google Scholar]
  4. Burton K. 1968 Determination of DNA concentration with diphenylamine. 163 166 In Grossman L., Moldave K. Methods in enzymology 12B Academic Press Inc.; New York:
    [Google Scholar]
  5. DeLey J., Tijtgat R. 1970; Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek. J. Microbiol. Serol 36 461 474
    [Google Scholar]
  6. Dellaglio F., Vescovo M., Premi L. 1974; “Free” diaminopimelic acid and deoxyribonucleic acid homology of Lactobacillus helveticus. Int. J. Syst. Bacteriol 24 235 237
    [Google Scholar]
  7. Gilmour M. N., Poole A. E. 1970; Growth stimulation of the mixed microbial flora of human dental plaques by haemin. Arch. Oral Biol 15 1343 1353
    [Google Scholar]
  8. Hine M. K., Berry G. P. 1937; Morphological and cultural studies on the genus Fusiformis. J. Bacteriol 34 517 534
    [Google Scholar]
  9. Hoffman H. 1957 Fusobacterium Knorr 436 440 In Breed R. S., Murray E. G. D., Smith N. R. Bergey’s manual of determinative bacteriology. , 7th. The Williams and Wilkins Co.; Baltimore:
    [Google Scholar]
  10. Hoyer B. H., McCullough N. B. 1968; Polynucleotide homologies of Brucella deoxyribonucleic acids. J. Bacteriol 95 444 448
    [Google Scholar]
  11. Jackson F. L., Goodman Y. E., Bel F. R., Wong P. C., Whitehours R. L. S. 1971; Taxonomic status of facultative and strictly anaerobic “corroding bacilli” that have been classified as Bacteroides corrodens. J. Med. Microbiol 4 171 184
    [Google Scholar]
  12. Jain K., Radsak K., Mannheim W. 1974; Differentiation of the Oxytocum group from Klebsiella by deoxyribonucleic acid-deoxyribonucleic acid hybridization. Int. J. Syst. Bacteriol 24 402 407
    [Google Scholar]
  13. Johnson J. L. 1973; Use of nucleic-acid homologies in the taxonomy of anaerobic bacteria. Int. J. Syst. Bacteriol 23 308 315
    [Google Scholar]
  14. Knorr M. 1922 Uber die fusospirilläre Symbiose, die Gattung Fusobacterium (K. B. Lehmann) und Spirillum sputigenum. (Zugleich ein Beitrag zur Bakteriologie der Mundhöhle) I. Mitteilung: die Epidemiologie der fusospirillaren Symbiose, besonders der Plaut-Vincentschen Angina. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. 89 536 545
  15. Knorr M. 1923 Uber die fusospirilläre Symbiose, die Gattung Fusobacterium (K. B. Lehmann) und Spirillum sputigenum. (Zugleich ein Beitrag zur Bakteriologie der Mundhölhle) II. Mitteilung: die Gattung Fusobacterium. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. 89 4 22
  16. Legault-Demare J., Desseaux B., Heyman T., Seror S., Ress G. P. 1967; Studies on hybrid molecules of nucleic acids. 1. DNA-DNA hybrids on nitrocellulose filters. Biochem. Biophys. Res. Commun 28 550 557
    [Google Scholar]
  17. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. Biol. Chem 193 265 275
    [Google Scholar]
  18. Mandel M., Marmur J. 1968 Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. 195 206 In Grossman L., Moldave K. Methods in enzymology 12B Academic Press Inc.; New York:
    [Google Scholar]
  19. Mandel M., Schildkraut C. L., Marmur J. 1968 Use of CsCl density gradient analysis for determining the guanine plus cytosine content of DNA. 184 195 In Grossman L., Moldave K. Methods in enzymology 12B Academic Press Inc.; New York:
    [Google Scholar]
  20. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol 3 208 218
    [Google Scholar]
  21. Moore W. E. C., Holdeman L. V. 1974 Fusobacterium Knorr 1922. 404 416 In Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology , 8th. The Williams and Wilkins Co.; Baltimore:
    [Google Scholar]
  22. Ritter D. B., Gerloff R. K. 1966; Deoxyribonucleic acid hybridization among some species of the genus Pasteurella. J. Bacteriol 92 1838 1839
    [Google Scholar]
  23. Roop D. R., Mundt J. O., Riggsby W. S. 1974; Deoxyribonucleic acid hybridization studies among some strains of group D and group N streptococci. Int. J. Syst. Bacteriol 24 330 337
    [Google Scholar]
  24. Slanetz L. W., Rettger L. F. 1933; A systematic study of the fusiform bacteria. J. Bacteriol 26 599 620
    [Google Scholar]
  25. Spaulding E. H., Rettger L. F. 1937; The Fusobacterium genus. I. Biochemical and serological classification. J. Bacteriol 34 535 548
    [Google Scholar]
  26. Varney P. 1927; The serological classification of fusiform bacilli. J. Bacteriol 13 275 314
    [Google Scholar]
  27. Werner H., Neuhaus F., Hussels H. 1971; A biochemical study of fusiform anaerobes. Med. Microbiol. Immunol 157 10 16
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-26-3-301
Loading
/content/journal/ijsem/10.1099/00207713-26-3-301
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error