1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 33, Issue 4

Cytochrome Oxidase Analyses of Strains: Existence of Oxidase-Positive Species Free

Abstract

Analyses of 173 strains (representing 30 species) with a newly developed quantitative colorimetric -tetramethyl--phenylenediamine oxidase assay revealed the presence of many oxidase-positive species. Of 144 mesophilic strains analyzed, the following were oxidase positive: 16 of 16 strains, 7 strains, 5 of 10 strains, 4 strains, 2 of 5 strains, 1 strain of and 1 strain of The thermophilic organisms T-10 and ATCC 8005, (one strain), and DSM 465 were also oxidase positive. In fact, when the oxidase-positive thermophilic strains were grown at 65°C, they exhibited very high oxidation rates; most of these rates were equivalent to the high rates of reaction recorded for strains of and spp. Most mesophilic strains and all of the psychrophiles examined were oxidase negative or oxidase indeterminate (the latter had oxidase rates equilavent to the rates in the -tetramethyl--phenylenediamine autooxidation reaction). Spectral absorbance analyses on membranes of oxidase-positive strain 385 revealed the presence of a type c cytochrome with cytochrome oxidases and + , strains which could not oxidize -tetramethyl--phenylenediamine lacked type cytochromes but possessed these two oxidases. The paper strip Kovacs oxidase test with Marion reagent could be routinely used for detecting oxidase-positive strains. A distinct class of oxidase-positive strains should be identified, which might eventually be useful for identification studies.

  • Published Online:
Copyright © 1983 International Union of Microbiological Societies
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-33-4-887
1983-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/33/4/ijs-33-4-887.html?itemId=/content/journal/ijsem/10.1099/00207713-33-4-887&mimeType=html&fmt=ahah

References

  1. Boswell P. A., Batstone G. F., Mitchell R. G. 1972; The oxidase reaction in the classification of the Micrococcaceae . J. Med. Microbiol 5:267–269
     [Google Scholar]
  2. Downs A. J., Jones C. W. 1975; Energy conservation in Bacillus megaterium . Arch. Microbiol 105:159–167
     [Google Scholar]
  3. Faller A., Schleifer K. H. 1981; Modified oxidase and benzidine tests for separation of staphylococci from micrococci. J. Clin. Miocrobiol 13:1031–1035
     [Google Scholar]
  4. Goodfellow M. 1968; Properties and composition of the bacterial flora of a pine forest soil. J. Soil Sci 19:154–167
     [Google Scholar]
  5. Gordon J., McLeod J. W. 1928; The practical application of the direct oxidase reaction in bacteriology. J. Pathol. Bacteriol 31:185–190
     [Google Scholar]
  6. Hollander R. 1977; The influence of permeability of the cell membrane on TMPD oxidase activity. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 237:351–357
     [Google Scholar]
  7. Jones C. W. 1980; Cytochrome patterns in classification and identification including their relevance to the oxidase test. 127–138 Goodfellow M., and Board R. G. Microbiological classification and identification Academic Press Inc.; New York:
     [Google Scholar]
  8. Jurtshuk P. Jr., McQuitty D. N. 1976; Survey of oxidase-positive and -negative bacteria using a quantitative Kovacs oxidase test. Int. J. Syst. Bacteriol 26:127–135
     [Google Scholar]
  9. Jurtshuk P. Jr., McQuitty D. N. 1976; Use of a quantitative oxidase test for characterizing oxidative metabolism in bacteria. Appl. Environ. Microbiol 31:668–679
     [Google Scholar]
  10. Jurtshuk P. Jr., Mueller T. J., Acord W. C. 1975; Bacterial terminal oxidases. Crit. Rev. Microbiol 3:399–468
     [Google Scholar]
  11. Jurtshuk P. Jr., Mueller T. J., McQuitty D. N., Riley W. H. 1978; The cytochrome oxidase reaction in Azotobacter vinelandii and other bacteria. 99–121 Degn H., Lloyd D., Hill G. C. Functions of alternative terminal oxidaseProceedings of the 11th FEBS Copenhagen Meeting Pergamon Press; Oxford:
     [Google Scholar]
  12. Jurtshuk P. Jr., Mueller T. J., Wong T. Y. 1981; Isolation and purification of the cytochrome oxidase of Azotobacter vinelandii . Biochim. Biophys. Acta 637:374–382
     [Google Scholar]
  13. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature (London) 178:703
     [Google Scholar]
  14. Lieberman M. M., Lanyi J. K. 1971; Studies on the electron transport chain of extremely halophilic bacteria. Biochim. Biophys. Acta 245:21–33
     [Google Scholar]
  15. Steel K. J. 1961; The oxidase reaction as a taxonomic tool. J. Gen. Microbiol 25:297–306
     [Google Scholar]
  16. Steel K. J. 1962; The oxidase activity of staphylococci. J. Appl. Microbiol 25:445–447
     [Google Scholar]
  17. Wolf J., Sharp R. J. 1981; Taxonomic and related aspects of thermophiles within the genus Bacillus . 251–296 Berkeley R. C. W., and Goodfellow M. The aerobic endospore forming bacteria Academic Press, Inc.; London:
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-33-4-887
Loading
Cytochrome Oxidase Analyses of Bacillus Strains: Existence of Oxidase-Positive Species
Int J Syst Evol Microbiol 33, 887 (1983); https://doi.org/10.1099/00207713-33-4-887
/content/journal/ijsem/10.1099/00207713-33-4-887
/content/journal/ijsem/10.1099/00207713-33-4-887
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error