1887

Abstract

The recently established species was examined for possible genetic and antigenic relatedness to the morphologically similar species and . The results of electrophoretic analysis of cell proteins, deoxyribonucleic acid (DNA) cleavage by restriction endonucleases, Southern blot analysis of digested mycoplasmal DNA with ribosomal ribonucleic acid genes and with total genomic DNA as probes, and Western immunoblot analysis rule out any significant degree of genetic relatedness between and the other two mycoplasmas, in contrast to the marked antigenic relatedness of to . The value of the above molecular tools in resolving taxonomic problems at the species level is demonstrated.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-38-2-147
1988-04-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/38/2/ijsem-38-2-147.html?itemId=/content/journal/ijsem/10.1099/00207713-38-2-147&mimeType=html&fmt=ahah

References

  1. Baseman J. B., Drouillard D. L., Leith D. K., Tully J. G. 1984; Absence of Mycoplasma pneumoniae cytadsorption protein Pl in Mycoplasma genitalium and Mycoplasma gallisepticum. Infect. Immun. 43:1103–1105
    [Google Scholar]
  2. Christiansen G., Christiansen C., Freundt E. A. 1985; Lack of genetic relatedness between Mycoplasma alvi and Mycoplasma sualvi. Int. J. Syst. Bacteriol. 35:210
    [Google Scholar]
  3. Del Giudice R. A., Tully J. G., Rose D. L., Cole R. M. 1985; Mycoplasma pirum sp. nov., a terminal structured mollicute from cell cultures. Int. J. Syst. Bacteriol. 35:285–291
    [Google Scholar]
  4. Hu P. C., Clyde W. A. Jr., Collier A. M. 1984; Conservation of pathogenic mycoplasma antigens. Isr. J. Med. Sci. 20:916–919
    [Google Scholar]
  5. Kenny G. E., Cartwright F. D. 1984; Immunoblotting for determination of the antigenic specificities of antibodies to the Mycoplasmatales. Isr. J. Med. Sci. 20:908–911
    [Google Scholar]
  6. Kirchhoff H., Beyene P., Fischer M., Flossdorf J., Heitmann J., Khattab B., Lopatta D., Rosengarten R., Seidel G., Yousef C. 1987; Mycoplasma mobile sp. nov., a new species from fish. Int. J. Syst. Bacteriol. 37:192–197
    [Google Scholar]
  7. Kirchhoff H., Rosengarten R., Lotz W., Fischer M., Lopatta D. 1984; Flask-shaped mycoplasmas: properties and pathogenicity for man and animals. Isr. J. Med. Sci. 20:848–853
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 226:680–685
    [Google Scholar]
  9. Leach R. H., Hales A., Furr P. M., Mitchelmore D. L., Taylor-Robinson D. 1987; Problems in the identification of Mycoplasma pirum isolated from human lymphoblastoid cell cultures. FEMS Microbiol. Lett. 44:293–297
    [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  11. Razin S. 1985; Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol. Rev. 49:419–455
    [Google Scholar]
  12. Razin S., Tully J. G.ed 1983 Methods in mycoplasmology 1 Academic Press, Inc.; New York:
    [Google Scholar]
  13. Towbin H., Staelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350–4354
    [Google Scholar]
  14. Tully J. G., Whitcomb R. F., Clark H. F., Williamson D. L. 1977; Pathogenic mycoplasmas: cultivation and vertebrate pathogenicity of a new spiroplasma. Science 195:892–894
    [Google Scholar]
  15. Yogev D., Razin S. 1986; Common deoxyribonucleic acid sequences in Mycoplasma genitalium and Mycoplasma pneumoniae genomes. Int. J. Syst. Bacteriol. 36:426–430
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-38-2-147
Loading
/content/journal/ijsem/10.1099/00207713-38-2-147
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error