1887

Abstract

Phenotypic data and phospholipid ester-linked fatty acid profiles indicate that pigmented bacterial strains isolated from a hypersaline Antarctic lake are members of the “flavobacterium-bacteroides” phylum and may represent new taxa. Nearly complete 16S rRNA sequences were obtained for representative strains by directly sequencing the polymerase chain reaction-amplified 16S rRNA gene. Sequence signatures confirmed that these organisms were members of the flavobacterium-bacteroides phylum. A phylogenetic analysis, in which the sequences of the Antarctic strains were compared with a large number of sequences available for members of the flavobacterium-bacteroides phylum, showed that the Antarctic strains were phylogenetically distinct. The new species cluster with a group of organisms that contains the type species of the genus Two new species are described, for which the names and are proposed; strains ACAM 44 (= DSM 5423) and ACAM 48 (= 5424) are the type strains of and , respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-43-1-77
1993-01-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/43/1/ijs-43-1-77.html?itemId=/content/journal/ijsem/10.1099/00207713-43-1-77&mimeType=html&fmt=ahah

References

  1. Bachmann B., Luke W., Hunsmann G. 1990; Improvement of PCR amplified DNA sequencing with the aid of detergents. Nucleic Acids Res 18:1309
    [Google Scholar]
  2. Dobson S. J., James S. R., Franzmann P. D., McMeekin T. A. 1991; A numerical taxonomic study of some pigmented bacteria isolated from Organic Lake, an Antarctic hypersaline lake. Arch. Microbiol 156:56–61
    [Google Scholar]
  3. Edwards U., Rogall T., Blöcker H., Emde M., Böttger E. C. 1989; Isolation and direct complete nucleotide determination of entire genes Characterization of a gene coding for 16S ribosomal genes. Nucleic Acids Res 17:7843–7853
    [Google Scholar]
  4. Embley T. M., Smida J., Stackebrandt E. 1988; Reverse transcriptase sequencing of 16S ribosomal RNA from Faenia rectivirgula, Pseudonocardia thermophila and Saccharopolys- pora hirsuta, three wall type IV actinomycetes which lack mycolic acids. J. Gen. Microbiol 134:961–966
    [Google Scholar]
  5. Felsenstein J. 1989; PHYLIP—phylogeny inference package(version 3.2). Cladistics 5:164–166
    [Google Scholar]
  6. Franzmann P. D., Tindall B. J. 1990; A chemotaxonomic study of members of the family Halomonadaceae. Syst. Appl. Microbiol 13:142–147
    [Google Scholar]
  7. Hanks P. (ed.) 1986; Collins dictionary of the English language 2nd ed. William Collins; Sydney:
    [Google Scholar]
  8. Hebert A. M., Vreeland R. H. 1987; Phenotypic comparison of halotolerant bacteria: Halomonas halodurans sp. nov., nom. rev., comb. nov. Int. J. Syst. Bacteriol 37:347–350
    [Google Scholar]
  9. Holmes B. 1991; The genera Flavobacterium, Sphingobacte- rium, and Weeksella. p. 3620–3630 In Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. (ed.) The prokaryotes, a handbook on the biology of bacteria: ecophysiology, isolation, identification, applications vol. 4 2nd ed. Springer-Verlag; New York:
    [Google Scholar]
  10. Holmes B., Owen R. J. 1979; Proposal that Flavobacterium breve be substituted as the type species of the genus in place of Flavobacterium aquatile and emended description of the genus Flavobacterium: status of the named species of Flavobacterium. Request for an opinion. Int. J. Syst. Bacteriol 29:416–426
    [Google Scholar]
  11. Holmes B., Owen R. J., McMeekin T. A. 1984; Genus Flavobacterium. p. 353–361 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1 The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  12. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc. Natl. Acad. Sci. USA 82:6955–6959
    [Google Scholar]
  13. Merkel J. R. 1972; Influence of salts on the vibriostatic action of 2,4-diamino-6,7-diisopropyl pteridine. Arch. Mikrobiol 81:379–382
    [Google Scholar]
  14. Olsen G. J., Larsen N., Woese C. R. 1991; The ribosomal RNA database project. Nucleic Acids Res 19:2017–2021
    [Google Scholar]
  15. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett. Appl. Microbiol 8:151–156
    [Google Scholar]
  16. Reichenbach H. 1989; Order 1. Cytophagales. p. 2010–2082 In Staley J. T., Bryant M. P., Pfennig N., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 3: The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  17. Rosenburg A. 1983; Pseudomonas halodurans sp. nov., a halotolerant bacterium. Arch. Microbiol 136:117–123
    [Google Scholar]
  18. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer- directed enzymatic amplification of DNA with thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467
    [Google Scholar]
  20. Skerratt J. H., Nichols P. D., Mancuso C. A., James S. R., Dobson S. J., McMeekin T. A., Burton H. 1991; The phospholipid ester-linked fatty acid composition of members of the family Halomonadaceae and genus Flavobacterium. A chemo- taxonomic guide. Syst. Appl. Microbiol 14:8–13
    [Google Scholar]
  21. Stackebrandt E. 1991; Unifying phylogeny and phenotypic diversity. p. 19–47 In Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. (ed.) The prokaryotes, a handbook on the biology of bacteria: ecophysiology, isolation, identification, applications vol. 1 2nd ed Springer-Verlag; New York:
    [Google Scholar]
  22. Stackebrandt E., Charfreitag O. 1990; Partial 16S rRNA primary structure of five Actinomyces species: phylogenetic implications and development of an Actinomyces israelii specific oligonucleotide probe. J. Gen. Microbiol 136:37–43
    [Google Scholar]
  23. Swofford D. L. 1990; PAUP: phylogenetic analysis using parsimony. version 3.0 Illinois Natural History Survey; Champaign:
    [Google Scholar]
  24. United States Biochemical Corporation 1989; Step-by-step protocols for DNA sequencing with Sequenase version 2.0. , 3rd ed. United States Biochemical Corp; Cleveland:
    [Google Scholar]
  25. Weisburg W. G., Oyaizu Y., Oyaizu H., Woese C. R. 1985; Natural relationship between bacteroides and flavobacteria. J. Bacteriol 164:230–236
    [Google Scholar]
  26. Woese C. R. 1987; Bacterial evolution. Microbiol. Rev 51:221–271
    [Google Scholar]
  27. Woese C. R., Maloy S., Mandelco L., Raj H. D. 1990; Phylogenetic placement of Spirosomaceae. Syst. Appl. Microbiol 13:19–23
    [Google Scholar]
  28. Woese C. R., Stackebrandt E., Macke T. J., Fox G. E. 1985; A phylogenetic definition of the major eubacterial taxa. Syst. Appl. Microbiol 6:143–151
    [Google Scholar]
  29. Woese C. R., Yang D., Mandelco L., Stetter K. O. 1990; The flexibacter-flavobacter connection. Syst. Appl. Microbiol 13:161–165
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-43-1-77
Loading
/content/journal/ijsem/10.1099/00207713-43-1-77
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error