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Volume 45, Issue 1

Heterogeneity of Genome Sizes within the Genus Spiroplasma Free

Abstract

Organisms belonging to the genus are currently classified into 23 groups, 17 of which have been assigned species epithets. We determined the genome sizes of representatives of 20 groups by using pulsed-field gel electrophoresis. Each genome size was deduced from the mobility of linear nonrestricted DNA, as well as from the sum of the sizes of restriction fragments obtained after digestion with I, a restriction endonuclease with a limited number of restriction sites in spiroplasma DNA. The values which we obtained indicated that the genome sizes of members of the genus range from 940 to 2,220 kbp.

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Copyright © 1995 International Union of Microbiological Societies
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1995-01-01
2024-05-18
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References

  1. Abalain-Colloc M. L., Chastel C., Tully J. G., Bove J. M., Whitcomb R. F., Gilot B., Williamson D. L. 1987; Spiroplasma sabaudiense sp. nov. from mosquitoes collected in France. Int. J. Syst. Bacteriol. 37:260–265
     [Google Scholar]
  2. Abalain-Colloc M. L., Rosen L., Tully J. G., Bove J. M., Chastel C., Williamson D. L. 1988; Spiroplasma taiwanense sp. nov., a new species from Culex tritaeniorhynchus mosquitoes collected in Taiwan. Int. J. Syst. Bacteriol. 38:103–107
     [Google Scholar]
  3. Abalain-Colloc M. L., Williamson D. L., Carle P., Abalain J. H., Tully J. G., Bonnet F., Konai M., Whitcomb R. F., Bove J. M., Chastel C. 1993; Division of group XVI spiroplasmas into subgroups. Int. J. Syst. Bacteriol. 43:342–346
     [Google Scholar]
  4. Clark T. B. 1982; Spiroplasmas: diversity of arthropod reservoirs and host-parasite relationships. Science 217:57–59
     [Google Scholar]
  5. Clark T. B. 1984; Diversity of spiroplasma host-parasite relationships. Isr. J. Med. Sci. 20:995–997
     [Google Scholar]
  6. Clark T. B., Henegar R. B., Rosen L., Hackett K. J., Whitcomb R. F., Lowry J. E., Saillard C., Bove J. M., Tully J. G., Williamson D. L. 1987; New spiroplasmas from insects and flowers: isolation, ecology, and host association. Isr. J. Med. Sci. 23:687–690
     [Google Scholar]
  7. Clark T. B., Peterson B. V., Whitcomb R. F., Henegar R. B., Hackett K. J., Tully J. G. 1984; Spiroplasmas in the Tabanidae . Isr. J. Med. Sci. 20:1002–1005
     [Google Scholar]
  8. Clark T. B., Whitcomb R. F., Tully J. G., Mouches C., Saillard C., Bove J. M., Wroblewski H., Carle P., Rose D. L., Henegar R. B., Williamson D. L. 1985; Spiroplasma melliferum, a new species from the honeybee (Apis mellifera). Int. J. Syst. Bacteriol. 35:296–308
     [Google Scholar]
  9. Colman S. D., Hu P. C., Litaker W., Bott K. F. 1990; A physical map of the Mycoplasma genitalium genome. Mol. Microbiol. 4:683–687
     [Google Scholar]
  10. Davis R. E., Lee I. M., Worley J. F. 1981; Spiroplasma florìcola, a new species isolated from surfaces of flowers of the tulip tree, Liriodendron tulipifera L. Int. J. Syst. Bacteriol. 31:456–464
     [Google Scholar]
  11. Eden-Green S. J., Waters H. 1981; Isolation and preliminary characterization of a spiroplasma from coconut palm in Jamaica. J. Gen. Microbiol. 124:263–270
     [Google Scholar]
  12. Gasparich G. E., Saillard C., Clark E. A., Konai M., French F. E., Tully J. G., Hackett K. J., Whitcomb R. F. 1993; Serologie and genomic relatedness of group VIII and group XVII spiroplasmas and subdivision of spiroplasma group VIII into subgroups. Int. J. Syst. Bacteriol. 43:338–341
     [Google Scholar]
  13. Grau O. 1991 Ph.D. thesis Université de Bordeaux II; Bordeaux, France:
     [Google Scholar]
  14. Grau O., Laigret F., Carle P., Tully J. G., Rose D. L., Bove J. M. 1991; Identification of a plant-derived mollicute as a strain of an avian pathogen, Mycoplasma iowae, and its implications for mollicute taxonomy. Int. J. Syst. Bacteriol. 41:473–478
     [Google Scholar]
  15. Guo Y. H., Chen T. A., Whitcomb R. F., Rose D. L., Tully J. G., Williamson D. L., Ye X. D., Chen Y. X. 1990; Spiroplasma chinense sp. nov. from flowers of Calystegia hederacea in China. Int. J. Syst. Bacteriol. 40:421–425
     [Google Scholar]
  16. Hackett K. J., Whitcomb R. F., Tully J. G., Rose D. L., Carle P., Bove J. M., Henegar R. B., Clark T. B., Clark E. A., Konai M., Adams J. R., Williamson D. L. 1993; Spiroplasma insolitum sp. nov., a new species of group I spiroplasma with an unusual DNA base composition. Int. J. Syst. Bacteriol. 43:272–277
     [Google Scholar]
  17. Hung S. Y., Chen T. A., Whitcomb R. F., Tully J. G., Chen X. Y. 1987; Spiroplasma culicicola sp. nov. from the salt marsh mosquito Aedes sollici-tans . Int. J. Syst. Bacteriol. 37:365–370
     [Google Scholar]
  18. Junca P., Saillard C., Tully J. G., Garcia-Jurado O., Degorce-Dumas J. R., Mouches C., Vignault J. C., Vogel R. 1980; Caractérisation de spiro-plasmes isolés d'insectes et de fleurs de France continentale, de Corse et du Maroc. Proposition pour une classification des spiroplasmes. C. R. Acad. Sci. Ser. D 290:1209–1212
     [Google Scholar]
  19. Kakulphimp J., Finch L. R., Robertson J. A. 1991; Genome sizes of mammalian and avian ureaplasmas. Int. J. Syst. Bacteriol. 41:326–327
     [Google Scholar]
  20. Konai M., Whitcomb R. F., Tully J. G., Rose D. L., Carle P., Bove J. M., Henegar R. B., Hackett K. J., Clark T. B., Williamson D. L. Spiroplasma velocicrescens sp. nov., a new species from the vespid wasp Monobia quadridens . Submitted for publication;
     [Google Scholar]
  21. Ladefoged S. A., Christiansen G. 1992; Physical and genetic mapping of the genomes of five Mycoplasma hominis strains by pulsed-field gel electro-phoresis. J. Bacteriol. 174:2199–2207
     [Google Scholar]
  22. Lei J. D., Su H. J., Chen T. A. 1979; Spiroplasmas isolated from green leaf bug Trigonotylus ruficornis Geoffroy. 89–97In Proceedings of the US-ROC Plant Mycoplasma Seminar. National Science Council; Taipei, Taiwan:
     [Google Scholar]
  23. Mouches C, Bove J. M., Tully J. G, Rose D. L., McCoy R. E, Carle Junca P., Gamier M., Saillard C. 1983; Spiroplasma apis, a new species from the honey bee Apis mellifera . Ann. Inst. Pasteur Microbiol. 134:383–397
     [Google Scholar]
  24. Neimark H. C, Lange C. S. 1990; Pulse-field electrophoresis indicates full-length mycoplasma chromosomes range widely in size. Nucleic Acids Res. 18:5443–5448
     [Google Scholar]
  25. Pickens E. G., Gerloff R. K., Burgdorfer W. 1968; Spirochete from the rabbit tick, HaemaphysalL· leporispalustris (Packard). J. Bacteriol. 95:291–299
     [Google Scholar]
  26. Pyle L. E., Corcoran L. N., Cocks B. J., Bergemann A. D., Whitley J. C., Finch L. R. 1988; Pulsed-field electrophoresis indicates larger than expected sizes for mycoplasma genomes. Nucleic Acids Res. 16:6015–6025
     [Google Scholar]
  27. Pyle L. E., Finch L. R. 1988; Preparation and FIGE separation of infrequent restriction fragments from Mycoplasma mycoides DNA. Nucleic Acids Res. 16:226–228
     [Google Scholar]
  28. Pyle L. E., Finch L. R. 1988; A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci. Nucleic Acids Res. 16:6027–6040
     [Google Scholar]
  29. Robertson J. A., Pyle L. E., Stemke G. W., Finch L. R. 1990; Human ureaplasmas show diverse sizes by pulsed-field electrophoresis. Nucleic Acids Res. 18:1451–1455
     [Google Scholar]
  30. Saglio P., Lhospital M., Lafleche D., Dupont G., Bove J. M., Tully J. G., Freundt E. A. 1973; Spiroplasma citri gen. and sp. n.: a mycoplasma-like organism associated with “stubborn” disease of citrus. Int. J. Syst. Bacteriol. 23:191–204
     [Google Scholar]
  31. Saillard C, Vignault J. C., Bove J. M., Raie A., Tully J. G., Williamson D. L., Fos A., Garnier M., Gadeau A., Carle P., Whitcomb R. F. 1987; Spiroplasma phoeniceum sp. nov., a new plant-pathogenic species from Syria. Int. J. Syst. Bacteriol. 37:106–115
     [Google Scholar]
  32. Schwartz D. C, Cantor C. R. 1984; Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37:67–75
     [Google Scholar]
  33. Smith C. L., Condemine G. 1990; New approaches for physical mapping of small genomes. J. Bacteriol. 172:1167–1172
     [Google Scholar]
  34. Smith C. L., Klco S., Cantor C. R. 1988; Pulsed field gel electrophoresis and the technology of large DNA molecules. 41–72In Davies K. Genome analysis: a practical approach IRL Press; Oxford:
     [Google Scholar]
  35. Su C. J., Baseman J. B. 1990; Genome size of Mycoplasma genitalium . J. Bacteriol. 172:4705–4707
     [Google Scholar]
  36. Tully J. G., Bove J. M., Laigret F., Whitcomb R. F. 1993; Revised taxonomy of the class Mollicutes: proposed elevation of a monophyletic cluster of arthropod-associated mollicutes to ordinal rank (Entomoplasma-tales, ord. nov.), with provision for familial rank to separate species with nonhelical morphology (Entomoplasmataceae, fam. nov.) from helical species (Spiroplasmataceae), and emended descriptions of the order Mycoplas-matales, family Mycoplasmataceae . Int. J. Syst. Bacteriol. 43:378–385
     [Google Scholar]
  37. Tully J. G., Rose D. L., Clark E., Carle P., Bove J. M., Henegar R. B., Whitcomb R. F., Colflesh D. E., Williamson D. L. 1987; Revised group classification of the genus Spiroplasma (class Mollicutes), with proposed new groups XII through XXIII. Int. J. Syst. Bacteriol. 37:357–364
     [Google Scholar]
  38. Tully J. G., Rose D. L., Yunker C. E., Carle P., Bove J. M., Williamson D. L., Whitcomb R. F. 1995; Spiroplasma ixodetis sp. nov., a new species from Ixodes pacificus ticks collected in Oregon. Int. J. Syst. Bacteriol. 45:23–28
     [Google Scholar]
  39. Tully J. G., Whitcomb R. F., Hackett K. J., Rose D. L., Henegar R. B., Bove J. M., Carle P., Williamson D. L., Clark T. B. 1994; Taxonomic descriptions of eight new non-sterol-requiring mollicutes assigned to the genus Mesoplasma . Int. J. Syst. Bacteriol. 44:685–693
     [Google Scholar]
  40. Tully J. G., Whitcomb R. F., Rose D. L., Bove J. M. 1982; Spiroplasma mirum, a new species from rabbit tick (Haemaphysalis leporispalustris). Int. J. Syst. Bacteriol. 32:92–100
     [Google Scholar]
  41. Tully J. G., Whitcomb R. F., Rose D. L., Bove J. M., Carle P., Somerson N. L., Williamson D. L., Eden-Green S. 1994; Acholeplasma brassicae sp. nov. and Acholeplasma palmae sp. nov., two non-sterol-requiring mollicutes from plant surfaces. Int. J. Syst. Bacteriol. 44:680–684
     [Google Scholar]
  42. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., Van Etten J., Maniloff J., Woese C. R. 1989; A phylogenetic analysis of the mycoplasmas: basis for their classification. J. Bacteriol. 171:6455–6467
     [Google Scholar]
  43. Whitcomb R. F. 1983; Culture media for spiroplasmas. 147–159In Razin S., and Tully J. G. Methods in mycoplasmology Academic Press; New York:
     [Google Scholar]
  44. Whitcomb R. F., Bove J. M., Chen T. A., Tully J. G., Williamson D. L. 1987; Proposed criteria for an interim serogroup classification for members of the genus Spiroplasma (class Mollicutes). Int. J. Syst. Bacteriol. 37:82–84
     [Google Scholar]
  45. Whitcomb R. F., Chastel C., Abalain-Colloc M., Stevens C., Tully J. G., Rose D. L., Carle P., Bove J. M., Henegar J. R. B., Hackett K. J., Clark T. B., Konai M., Williamson D. L. 1993; Spiroplasma cantharicola sp. nov., from cantharid beetles (Coleoptera: Cantharidae). Int. J. Syst. Bacteriol. 43:421–424
     [Google Scholar]
  46. Whitcomb R. F., Chen T. A., Williamson D. L., Liao C., Tully J. G., Bove J. M., Mouches C., Rose D. L., Coan M. E., Clark T. B. 1986; Spiroplasma kunkelii sp. nov.: characterization of the etiological agent of corn stunt disease. Int. J. Syst. Bacteriol. 36:170–178
     [Google Scholar]
  47. Whitcomb R. F., Tully J. G., Rose D. L., Carle P., Bove J. M., Henegar R. B., Hackett K. J., Clark T. B., Konai M., Adams J., Williamson D. L. 1993; Spiroplasma monobiae sp. nov. from the vespid wasp Monobia quadridens (Hymenoptera: Vespidae). Int. J. Syst. Bacteriol. 43:256–260
     [Google Scholar]
  48. Whitcomb R. F., Vignault J. C., Tully J. G., Rose D. L., Carle P., Bove J. M., Hackett K. J., Henegar R. B., Konai M., Williamson D. L. 1993; Spiroplasma clarkii sp. nov. from the green June beetle (Coleoptera: Scar-abaeidae). Int. J. Syst. Bacteriol. 43:261–265
     [Google Scholar]
  49. Whitley J. C. 1991 Ph.D. thesis University of Melbourne; Melbourne, Australia:
     [Google Scholar]
  50. Williamson D. L., Tully J. G., Whitcomb R. F. 1989; The genus Spiroplasma . 71–111In Barile M. F., Razin S., Tully J. G., Whitcomb R. F. The mycoplasmas 5 Academic Press, Inc.; San Diego, Calif:
     [Google Scholar]
  51. Ye F. Ph.D. thesis. Université de Bordeaux II; Bordeaux, France:
  52. Ye F., Laigret F., Bove J. M. 1994; A physical map of the prokaryote Spiroplasma melliferum and its comparison with the Spiroplasma citri map. C. R. Acad. Sci. 317:392–398
     [Google Scholar]
  53. Ye F., Laigret F., Whitley J. C., Citti C., Finch L. R., Carle P., Renaudin J., Bove J. M. 1992; A physical and genetic map of the Spiroplasma citri genome. Nucleic Acids Res. 20:1559–1565
     [Google Scholar]
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Heterogeneity of Genome Sizes within the Genus Spiroplasma
Int J Syst Evol Microbiol 45, 178 (1995); https://doi.org/10.1099/00207713-45-1-178
/content/journal/ijsem/10.1099/00207713-45-1-178
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