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- Volume 65, Issue 8
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f Humibacter ginsengiterrae sp. nov., and Humibacter ginsengisoli sp. nov., isolated from soil of a ginseng field
- Authors: Eul-Kon Kim1,3 , Van-An Hoang1,3 , Yeon-Ju Kim1 , Ngoc-Lan Nguyen1 , Johan Sukweenadhi2 , Jong-Pyo Kang1 , Deok-Chun Yang1,2
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1 1Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea 2 2Graduate school of Biotechnology, Ginseng Genetic Resource Bank, College of life science, Kyung Hee University, Yongin, 446-701, Republic of Korea
- Correspondence Deok-Chun Yang [email protected]
- First Published Online: 01 August 2015, International Journal of Systematic and Evolutionary Microbiology 65: 2734-2740, doi: 10.1099/ijs.0.000335
- Subject: NEW TAXA - Bacteroidetes
- Cover date:




Humibacter ginsengiterrae sp. nov., and Humibacter ginsengisoli sp. nov., isolated from soil of a ginseng field, Page 1 of 1
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Two novel Gram-staining-positive bacteria, designated DCY60T and DCY90T, were isolated from soil of a ginseng field in the Republic of Korea. 16S rRNA gene sequence comparisons showed the two novel strains were closely related to members of the genus Humibacter with greatest similarity to Humibacter antri KCTC 33009T (98.8 and 98.4 % for DCY60T and DCY90T, respectively). The predominant menaquinones present were MK-11 and MK-12. The major fatty acids were anteiso-C17 : 0 and summed feature 8 containing C18 : 1ω7c and/or C18 : 1ω6c. The DNA G+C contents of strains DCY60T and DCY90T were 62.8 and 66.8 mol%, respectively. The peptidoglycan of both strains contained the amino acids ornithine, 2,4-diaminobutyric acid, alanine, glutamic acid and glycine. The cell-wall sugars of strain DCY60T comprised glucose, galactose, rhamnose and xylose, while strain DCY90T contained glucose, galactose, rhamnose and ribose. The major polar lipids of both strains were phosphatidylglycerol, an unidentified glycolipid, and an unknown phospholipid. On the basis of the phenotypic analysis strains DCY60T and DCY90T represent novel species of the genus Humibacter, for which names Humibacter ginsengiterrae sp. nov. (type strain DCY60T = KCTC 33520T = JCM 30079T) and Humibacter ginsengisoli sp. nov. (type strain DCY90T = KCTC 33521T = JCM 30080T) are proposed.
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† These authors contributed equally to this manuscript.
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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Humibacter ginsengiterrae sp. nov. DCY60T and Humibacter ginsengisoli sp. nov. DCY90T are JQ010859 and KF915800, respectively.
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Three supplementary figures are available with the online Supplementary Material.
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Abbreviations: ML maximum-likelihood MP maximum-parsimony NJ neighbour-joining
© 2015 IUMS | Published by the Microbiology Society
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