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- Volume 65, Issue 9
- Article

f Marinobacter halophilus sp. nov., a halophilic bacterium isolated from a salt lake
- Authors: Zhi-Ping Zhong1,2 , Ying Liu1 , Hong-Can Liu3 , Fang Wang4 , Yu-Guang Zhou3 , Zhi-Pei Liu1
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- VIEW AFFILIATIONS
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1 1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China 2 2University of Chinese Academy of Sciences, Beijing 100049, PR China 3 3China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China 4 4State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, China Institute of Water Resources and Hydropower Research, Beijing 100089, PR China
- Correspondence Zhi-Pei Liu [email protected]
- First Published Online: 01 September 2015, International Journal of Systematic and Evolutionary Microbiology 65: 2838-2845, doi: 10.1099/ijs.0.000338
- Subject: NEW TAXA - Proteobacteria
- Cover date:




Marinobacter halophilus sp. nov., a halophilic bacterium isolated from a salt lake, Page 1 of 1
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A Gram-staining-negative bacterium, strain XCD-X12T, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9 %, w/w) in Qaidam basin, Qinghai Province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X12T were non-spore-forming rods, 0.4–0.7 μm wide, 2.1–3.2 μm long and motile with a single polar flagellum. Strain XCD-X12T was strictly aerobic and catalase- and oxidase-positive. Growth was observed in the presence of 0–20.0 % (w/v) NaCl (optimum, 4.0–8.0 %), at 4–35 °C (optimum, 30 °C) and at pH 6.5–10.5 (optimum, pH 8.5). It contained Q-9 as the predominant respiratory quinone. The major fatty acids (>10.0 %) were C16 : 0, C16 : 1ω9c and C18 : 1ω9c. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and an uncharacterized aminophospholipid. The DNA G+C content was 55.6 mol% (T m). Phylogenetic trees based on 16S rRNA gene sequences showed that strain XCD-X12T was associated with the genus Marinobacter, and showed the highest 16S rRNA gene sequence similarity to Marinobacter hydrocarbonoclasticus ATCC 49840T (97.4 %), M. vinifirmus FB1T (96.8 %), M. excellens KMM 3809T (96.8 %) and M. antarcticus ZS2-30T (96.7 %). DNA–DNA relatedness of strain XCD-X12T to M. hydrocarbonoclasticus CGMCC 1.7683T was 34 ± 5 %. Based on these data, it is concluded that strain XCD-X12T represents a novel species of the genus Marinobacter, for which the name Marinobacter halophilus sp. nov. is proposed. The type strain is XCD-X12T ( = CGMCC 1.12481T = JCM 30472T).
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain XCD-X12T is KC762322.
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Two supplementary figures are available with the online Supplementary Material.
© 2015 IUMS | Published by the Microbiology Society
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