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f sp. nov., a halophilic bacterium isolated from a salt lake

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  • Authors: Zhi-Ping Zhong1​,2​ ,  Ying Liu1​ ,  Hong-Can Liu3​ ,  Fang Wang4​ ,  Yu-Guang Zhou3​ , Zhi-Pei Liu1​
    • VIEW AFFILIATIONS
    • 1​ 1​State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China 2​ 2​University of Chinese Academy of Sciences, Beijing 100049, PR China 3​ 3​China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China 4​ 4​State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, China Institute of Water Resources and Hydropower Research, Beijing 100089, PR China
  • Zhi-Pei Liu [email protected]
  • First Published Online: 01 September 2015, International Journal of Systematic and Evolutionary Microbiology 65: 2838-2845, doi: 10.1099/ijs.0.000338
  • Subject: NEW TAXA - Proteobacteria
  • Cover date:
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  • A Gram-staining-negative bacterium, strain XCD-X12, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9 %, w/w) in Qaidam basin, Qinghai Province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X12 were non-spore-forming rods, 0.4–0.7 μm wide, 2.1–3.2 μm long and motile with a single polar flagellum. Strain XCD-X12 was strictly aerobic and catalase- and oxidase-positive. Growth was observed in the presence of 0–20.0 % (w/v) NaCl (optimum, 4.0–8.0 %), at 4–35 °C (optimum, 30 °C) and at pH 6.5–10.5 (optimum, pH 8.5). It contained Q-9 as the predominant respiratory quinone. The major fatty acids (>10.0 %) were C, Cω9 and Cω9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and an uncharacterized aminophospholipid. The DNA G+C content was 55.6 mol% ( ). Phylogenetic trees based on 16S rRNA gene sequences showed that strain XCD-X12 was associated with the genus , and showed the highest 16S rRNA gene sequence similarity to ATCC 49840 (97.4 %), FB1 (96.8 %), KMM 3809 (96.8 %) and ZS2-30 (96.7 %). DNA–DNA relatedness of strain XCD-X12 to CGMCC 1.7683 was 34 ± 5 %. Based on these data, it is concluded that strain XCD-X12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XCD-X12 ( = CGMCC 1.12481 = JCM 30472).

  • The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain XCD-X12 is KC762322.

  • Two supplementary figures are available with the online Supplementary Material.

© 2015 IUMS | Published by the Microbiology Society

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