1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 12

sp. nov a taurocholic acid-producing bacterium isolated from the marine ascidian Free

Abstract

A marine bacterium, strain KME 001, was isolated from the siphon tissue of a marine ascidian, , collected off the coast of Gangneung, Korea. Strain KME 001 was a Gram-positive, aerobic, non-spore-forming, rod-shaped and non-motile bacterium. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain KME 001 clustered with the genus and was closely related to , and with 97.7, 97.6 and 97.5 % sequence similarities, respectively. The strain was capable of growth at a variety of temperatures (10–42 °C) and over a broad pH range (5.0–10.0). NaCl was required for robust growth of the strain. The diagnostic diamino acid of the cell-wall peptidoglycan was -diaminopimelic acid. The predominant menaquinone was MK-9(H). The predominant fatty acids were C 9, C and 10-methyl C. The DNA–DNA hybridization analyses showed that DNA–DNA relatedness values between strain KME 001 and its nearest neighbours, KCTC 19207, KCCM 41104 and KACC 20565, were 49.6, 57.1 and 63.5 %, respectively. The DNA G+C content of strain KME 001 was 75.9 mol%. Chemical investigation of the liquid culture medium of strain KME 001 led to the isolation of taurocholic acid as a major secondary metabolite. On the basis of phylogenetic and phenotypic data, strain KME 001 is classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KME 001 (=JCM 15749=KCCM 90079).

  • Published Online:
Keyword(s): SEM, scanning electron microscope
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.016618-0
2010-12-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/12/2793.html?itemId=/content/journal/ijsem/10.1099/ijs.0.016618-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
     [Google Scholar]
  2. Bruns A., Philipp H., Cypionka H., Brinkhoff T. 2003; Aeromicrobium marinum sp. nov., an abundant pelagic bacterium isolated from the German Wadden Sea. Int J Syst Evol Microbiol 53:1917–1923 [CrossRef]
     [Google Scholar]
  3. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
     [Google Scholar]
  4. Collins M. D., Stackebrandt E. 1989; Molecular taxonomic studies on some LL-diaminopimelic acid-containing coryneforms from herbage: description of Nocardioides fastidiosa sp. nov. FEMS Microbiol Lett 48:289–293
     [Google Scholar]
  5. Cui Y. S., Im W. T., Yin C. R., Lee J. S., Lee K. C., Lee S. T. 2007; Aeromicrobium panaciterrae sp. nov., isolated from soil of a ginseng field in South Korea. Int J Syst Evol Microbiol 57:687–691 [CrossRef]
     [Google Scholar]
  6. Dewick P. M. 2001; Medicinal natural products: a biosynthetic approach. In The Mevalonate and Deoxyxylulose Phosphate Pathways: Terpenoids and Steroids. , 2nd edn. pp 260–262 West Sussex, UK: Wiley;
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
     [Google Scholar]
  8. Gowda N. G. A., Ijare O. B., Somashekar B. S., Sharma A., Kapoor V. K., Khetrapal C. L. 2006; Single-step analysis of individual conjugated bile acids in human bile using 1H NMR spectroscopy. Lipids 41:591–603 [CrossRef]
     [Google Scholar]
  9. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
     [Google Scholar]
  10. Ijare O. B., Somashekar B. S., Jadegoud Y., Gowda N. G. A. 2005; 1H and 13C NMR characterization and stereochemical assignments of bile acids in aqueous media. Lipids 40:1031–1041 [CrossRef]
     [Google Scholar]
  11. Katayama-Fujimura Y., Komatsu Y., Kuraishi H., Kaneko T. 1984; Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. Agric Biol Chem 48:3169–3172 [CrossRef]
     [Google Scholar]
  12. Kim D., Lee J. S., Kim J., Kang S.-J., Yoon J.-H., Kim W. G., Lee C. H. 2007; Biosynthesis of bile acids in a variety of marine bacterial taxa. J Microbiol Biotechnol 17:403–407
     [Google Scholar]
  13. Kim M. K., Park M.-J., Im W.-T., Yang D.-C. 2008; Aeromicrobium ginsengisoli sp. nov., isolated from a ginseng field. Int J Syst Evol Microbiol 58:2025–2030 [CrossRef]
     [Google Scholar]
  14. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
     [Google Scholar]
  15. Kumar S., Nei M., Dudley J., Tamura K. 2008; mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306 [CrossRef]
     [Google Scholar]
  16. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
     [Google Scholar]
  17. Lee S. D., Kim S. J. 2007; Aeromicrobium tamlense sp. nov., isolated from dried seaweed. Int J Syst Evol Microbiol 57:337–341 [CrossRef]
     [Google Scholar]
  18. Lee D. W., Lee S. D. 2008; Aeromicrobium ponti sp. nov., isolated from seawater. Int J Syst Evol Microbiol 58:987–991 [CrossRef]
     [Google Scholar]
  19. Maneerat S., Nitoda T., Kanzaki H., Kawai F. 2005; Bile acids are new products of a marine bacterium, Myroides sp. strain SM1. Appl Microbiol Biotechnol 67:679–683 [CrossRef]
     [Google Scholar]
  20. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586
     [Google Scholar]
  21. Miller E. S., Woese C. R., Brenner S. 1991; Description of the erythromycin-producing bacterium Arthrobacter sp. strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp. nov.. Int J Syst Bacteriol 41:363–368 [CrossRef]
     [Google Scholar]
  22. Park S.-C., Kim C.-J., Uramoto M., Yun H. I., Yoon K.-H., Oh T.-K. 1995; Antibacterial substance produced by Streptococcus faecium under anaerobic culture. Biosci Biotechnol Biochem 59:1966–1967 [CrossRef]
     [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  24. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
     [Google Scholar]
  25. Süßmuth R., Eberspächer J., Haag R., Springer W. 1987 Biol-chemischmikrobiologisches Praktikum Stuttgart: Thieme Verlag;
     [Google Scholar]
  26. Tamaoka J. 1986; Analysis of bacterial menaquinone mixtures by reverse-phase high-performance liquid chromatography. Methods Enzymol 123:251–256
     [Google Scholar]
  27. Tamura T., Yokota A. 1994; Transfer of Nocardioides fastidiosa Collins and Stackebrandt 1989 to the genus Aeromicrobium as Aeromicrobium fastidiosum comb. nov. Int J Syst Bacteriol 44:608–611 [CrossRef]
     [Google Scholar]
  28. Tang Y., Zhou G., Zhang L., Mao J., Luo X., Wang M., Fang C. 2008; Aeromicrobium flavum sp. nov., isolated from air. Int J Syst Evol Microbiol 58:1860–1863 [CrossRef]
     [Google Scholar]
  29. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
     [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  31. Yoon J.-H., Lee C.-H., Oh T. K. 2005; Aeromicrobium alkaliterrae sp. nov., isolated from an alkaline soil, and emended description of the genus Aeromicrobium . Int J Syst Evol Microbiol 55:2171–2175 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.016618-0
Loading
Aeromicrobium halocynthiae sp. nov., a taurocholic acid-producing bacterium isolated from the marine ascidian Halocynthia roretzi
Int J Syst Evol Microbiol 60, 2793 (2010); https://doi.org/10.1099/ijs.0.016618-0
/content/journal/ijsem/10.1099/ijs.0.016618-0
/content/journal/ijsem/10.1099/ijs.0.016618-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error