1887

Abstract

A novel actinomycete, designated strain OU-63, was isolated from garden soil collected on the campus of Osmania University in Hyderabad, southern India. The strain was found to have morphological and chemotaxonomic characteristics typical of the genus . Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belonged to the genus , and was related most closely to NBRC 12753 (98.6 % similarity). However, the mean level of DNA–DNA relatedness between the two strains was only 25±1.73 %. Based on DNA–DNA relatedness, morphological and phenotypic data, strain OU-63 could be distinguished from the type strains of phylogenetically related species. It is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is OU-63 (=CCTCC AA209025 =PCM 2690).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.017749-0
2010-08-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/8/1755.html?itemId=/content/journal/ijsem/10.1099/ijs.0.017749-0&mimeType=html&fmt=ahah

References

  1. Anderson A. S., Wellington E. M. H. 2001; The taxonomy of Streptomyces and related genera. Int J Syst Evol Microbiol 51:797–814 [CrossRef]
    [Google Scholar]
  2. Atalan E., Manfio G. P., Ward A. C., Kroppenstedt R. M., Goodfellow M. 2000; Biosystematic studies on novel streptomycetes from soil. Antonie van Leeuwenhoek 77:337–353 [CrossRef]
    [Google Scholar]
  3. Berdy J. 2005; Bioactive microbial metabolites. J Antibiot 58:1–26 [CrossRef]
    [Google Scholar]
  4. Chung Y. R., Sung K. C., Mo H. K., Son D. Y., Nam J. S., Chun J. S., Bae K. S. 1999; Kitasatospora cheerisanensis sp. nov., a new species of the genus Kitasatospora that produces an antifungal agent. Int J Syst Bacteriol 49:753–758 [CrossRef]
    [Google Scholar]
  5. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  6. Euzéby J. P. 2009 List of Prokaryotic Names with Standing in Nomenclature ; http://www.bacterio.cict.fr/
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  8. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  9. Gonzalez J. M., Saiz-Jimenez C. 2005; A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures. Extremophiles 9:75–79 [CrossRef]
    [Google Scholar]
  10. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  12. Kawato M., Shinobu R. 1959; On Streptomyces herbaricolor sp. nov., supplement: a single technique for microscopical observation. Mem Osaka Unit Lib Arts Educ B Nat Sci 8:114–119
    [Google Scholar]
  13. Kim S. B., Goodfellow M. 2002; Streptomyces avemitilis sp. , nov., nom. rev. a taxonomic home for the avermectin-producing streptomycetes. Int J Syst Evol Microbiol 521225–1228 [CrossRef]
    [Google Scholar]
  14. Kim S. B., Lonsdale J., Seong C. N., Goodfellow M. 2003; Streptacidiphilus gen. nov., acidophilic actinomycetes with wall chemotype I and emendation of the family Streptomycetaceae (Waksman & Henrici 1943AL) emend. Rainey et al. (1997). Antonie van Leeuwenhoek 83:107–116 [CrossRef]
    [Google Scholar]
  15. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001 mega2: Molecular Evolutionary Genetics Analysis software Tempe, AZ: Arizona State University;
    [Google Scholar]
  16. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  18. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy pp 227–291 Edited by Dietz A., Thayer D. W. Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  19. Lepage G., Roy C. C. 1984; Improved recovery of fatty acid through direct transesterification without prior extraction or purification. J Lipid Res 25:1391–1396
    [Google Scholar]
  20. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  21. Manfio G. P., Zakrzewska-Czerwinska J., Atalan E., Goodfellow M. 1995; Towards minimal standards for the description of Streptomyces species. Biotechnologiia 8:228–237
    [Google Scholar]
  22. Manfio G. P., Atalan E., Zakrzewska-Czerwinska J., Mordarski M., Rodríguez C., Collins M. D., Goodfellow M. 2003; Classification of novel soil streptomycetes as Streptomyces aureus sp.nov., Streptomyces laceyi sp. nov. and Streptomyces sanglieri sp. nov. Antonie van Leeuwenhoek 83:245–255 [CrossRef]
    [Google Scholar]
  23. Olson E. H. 1968; Actinomycetes Isolation Agar Supplementary Literature . Detroit, MI: Difco Laboratories;
    [Google Scholar]
  24. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [CrossRef]
    [Google Scholar]
  25. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  26. Sembiring L., Ward A. C., Goodfellow M. 2000; Selective isolation and characterisation of members of the Streptomyces violaceusniger clade associated with roots of Paraserianthes falcataria . Antonie van Leeuwenhoek 78:353–366 [CrossRef]
    [Google Scholar]
  27. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  28. Shirling E. B., Gottlieb D. 1968; Cooperative description of type cultures of Streptomyces . II. Species descriptions from first study. Int J Syst Bacteriol 18:69–189 [CrossRef]
    [Google Scholar]
  29. Shirling E. B., Gottlieb D. 1972; Cooperative description of type cultures of Streptomyces . V. Additional descriptions. Int J Syst Bacteriol 22:265–394 [CrossRef]
    [Google Scholar]
  30. Tripathi G., Rawal S. K. 1998; A simple and efficient protocol for isolation of high molecular weight DNA from Streptomyces aureofaciens . Biotechnol Tech 12:629–631 [CrossRef]
    [Google Scholar]
  31. Waksman S. A., Henrici A. T. 1943; The nomenclature and classification of the actinomycetes. J Bacteriol 46:337–341
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  33. Williams S. T., Davies F. L. 1967; Use of scanning electron microscope for the examination of actinomycetes. J Gen Microbiol 48:171–177 [CrossRef]
    [Google Scholar]
  34. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  35. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey's Manual of Systematic Bacteriology vol. 4 pp 2452–2492 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  36. Zhou Z. H., Liu Z. H., Qiao Y. D., Kim S. B., Goodfellow M. 1998; Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48:53–58 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.017749-0
Loading
/content/journal/ijsem/10.1099/ijs.0.017749-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error