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Abstract

Strains RU-16, RU-28, RU-04 and PU-02 were isolated from the gut of the African mole cricket, . Phylogenetic analyses based on 16S rRNA gene sequences revealed that the strains belonged to the family . All four strains were most closely related to DCY26 (below 97 % 16S rRNA gene sequence similarity). These isolates were Gram-stain-positive, motile (by gliding), rod-shaped and exhibited ivory-coloured colonies. Their chemotaxonomic properties included MK-11 as the major respiratory quinone, ornithine as the cell-wall diamino acid, acetyl as the acyl type of the peptidoglycan, cyclohexyl-C as the major fatty acid and phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids. On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, we propose a new genus in the family , gen. nov., with three novel species, sp. nov. (type strain RU-04  = KCTC 13809  = JCM 17590), sp. nov. (type strain RU-16  = KCTC 13810  = JCM 17591) and sp. nov. (type strain PU-02  = KCTC 13808  = JCM 17593). is the type species of the genus. Additionally, we propose that should be reclassified in the genus as comb. nov. (type strain DCY26  = KCTC 13163  = JCM 14773).

Funding
This study was supported by the:
  • KRIBB research initiative program (Award KGM0231133)
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2012-10-01
2024-04-28
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References

  1. Aizawa T., Ve N. B., Kimoto K., Iwabuchi N., Sumida H., Hasegawa I., Sasaki S., Tamura T., Kudo T. other authors 2007; Curtobacterium ammoniigenes sp. nov., an ammonia-producing bacterium isolated from plants inhabiting acidic swamps in actual acid sulfate soil areas of Vietnam. Int J Syst Evol Microbiol 57:1447–1452 [View Article][PubMed]
    [Google Scholar]
  2. Behrendt U., Ulrich A., Schumann P., Naumann D., Suzuki K. 2002; Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of Subtercola pratensis sp. nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov., sp. nov.. Int J Syst Evol Microbiol 52:1441–1454 [View Article][PubMed]
    [Google Scholar]
  3. Behrendt U., Schumann P., Hamada M., Suzuki K., Spröer C., Ulrich A. 2011; Reclassification of Leifsonia ginsengi (Qiu et al. 2007) as Herbiconiux ginsengi gen. nov., comb. nov. and description of Herbiconiux solani sp. nov., an actinobacterium associated with the phyllosphere of Solanum tuberosum L.. Int J Syst Evol Microbiol 61:1039–1047 [View Article][PubMed]
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  5. Breznak J. A. 1982; Intestinal microbiota of termites and other xylophagous insects. Annu Rev Microbiol 36:323–343 [View Article][PubMed]
    [Google Scholar]
  6. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  7. Dillon R. J., Dillon V. M. 2004; The gut bacteria of insects: nonpathogenic interactions. Annu Rev Entomol 49:71–92 [View Article][PubMed]
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  10. Felsenstein J. 2005 phylip (Phylogeny Inference Package), version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. Kim M. K., Kim Y. J., Kim H. B., Kim S. Y., Yi T. H., Yang D. C. 2008; Curtobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 58:2393–2397 [View Article][PubMed]
    [Google Scholar]
  12. Kim Y., Han M. K., Park D. S., Lee J. S., Oh H. W., Shin D. H., Jeong T. S., Kim S. U., Bae K. S. other authors 2009; Novel GH10 xylanase, with a fibronectin type 3 domain, from Cellulosimicrobium sp. strain HY-13, a bacterium in the gut of Eisenia fetida . Appl Environ Microbiol 75:7275–7279 [View Article][PubMed]
    [Google Scholar]
  13. Kim B.-C., Park D.-S., Kim H., Oh H.-W., Lee K. H., Shin K.-S., Bae K. S. 2012; Herbiconiux moechotypicola sp. nov., a xylanolytic bacterium isolated from the gut of hairy long-horned toad beetles, Moechotypa diphysis (Pascoe). Int J Syst Evol Microbiol 62:90–95 [View Article][PubMed]
    [Google Scholar]
  14. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  15. Lemke T., Stingl U., Egert M., Friedrich M. W., Brune A. 2003; Physicochemical conditions and microbial activities in the highly alkaline gut of the humus-feeding larva of Pachnoda ephippiata (Coleoptera: Scarabaeidae). Appl Environ Microbiol 69:6650–6658 [View Article][PubMed]
    [Google Scholar]
  16. Lilburn T. G., Kim K. S., Ostrom N. E., Byzek K. R., Leadbetter J. R., Breznak J. A. 2001; Nitrogen fixation by symbiotic and free-living spirochetes. Science 292:2495–2498 [View Article][PubMed]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  18. Park Y. H., Suzuki K., Yim D. G., Lee K. C., Kim E., Yoon J., Kim S., Kho Y. H., Goodfellow M., Komagata K. 1993; Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA. Antonie van Leeuwenhoek 64:307–313 [View Article][PubMed]
    [Google Scholar]
  19. Park D. S., Oh H. W., Jeong W. J., Kim H. M., Park H. Y., Bae K. S. 2007; A culture-based study of the bacterial communities within the guts of nine longicorn beetle species and their exo-enzyme producing properties for degrading xylan and pectin. J Microbiol 45:394–401[PubMed]
    [Google Scholar]
  20. Qiu F., Huang Y., Sun L., Zhang X., Liu Z., Song W. 2007; Leifsonia ginsengi sp. nov., isolated from ginseng root. Int J Syst Evol Microbiol 57:405–408 [View Article][PubMed]
    [Google Scholar]
  21. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Sasser M. 2001; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  23. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  24. Suzuki K., Sasaki J., Uramoto M., Nakase T., Komagata K. 1997; Cryobacterium psychrophilum gen. nov., sp. nov., nom. rev., comb. nov., an obligately psychrophilic actinomycete to accommodate “Curtobacterium psychrophilum” Inoue and Komagata 1976. Int J Syst Bacteriol 47:474–478 [View Article][PubMed]
    [Google Scholar]
  25. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  27. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [View Article]
    [Google Scholar]
  28. Vaz-Moreira I., Nobre M. F., Ferreira A. C. S., Schumann P., Nunes O. C., Manaia C. M. 2008; Humibacter albus gen. nov., sp. nov., isolated from sewage sludge compost. Int J Syst Evol Microbiol 58:1014–1018 [View Article][PubMed]
    [Google Scholar]
  29. Warnecke F., Luginbühl P., Ivanova N., Ghassemian M., Richardson T. H., Stege J. T., Cayouette M., McHardy A. C., Djordjevic G. other authors 2007; Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite. Nature 450:560–565 [View Article][PubMed]
    [Google Scholar]
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