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Volume 62, Issue Pt_9

sp. nov., a swarming, moderately halotolerant bacterium isolated from the intestinal tract of an earthworm ( L.) Free

Abstract

A swarming and moderately halotolerant bacterium, designated strain A1-2, was isolated from the intestinal tract of the earthworm L. Cells were endospore-forming rods that were facultatively anaerobic, catalase-positive, oxidase-negative and motile by peritrichous flagella. The isolate grew optimally at 30 °C and pH 7.0, and could grow with up to 9 % (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain A1-2 belonged to the genus and exhibited 16S rRNA gene sequence similarities of 96.8, 96.0, 96.0, 96.4 and 96.7 % with LMG 21831, PT-45, BAC 1015, SMC 4352-1 and LMG 18435, respectively. DNA–DNA relatedness values between the isolate and the reference strains were ≤38.3 % The DNA G+C content of strain A1-2 was 38.5 mol%. The predominant menaquinone was MK-7 and the major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acids were iso-C (51.5 %) and anteiso-C (29.6 %) and the cell-wall diamino acid was -diaminopimelic acid. On the basis of 16S rRNA gene sequence analysis and chemotaxonomic and phenotypic characteristics, it is concluded that strain A1-2 represents a novel species of the genus , for which we propose the name sp. nov. The type strain is A1-2 ( = KCCM 90092 = JCM 16993).

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Funding
This study was supported by the:
  • Basic Science Research Program
  • National Research Foundation of Korea (NRF)
  • Ministry of Education, Science and Technology
  • Yonsei University Research Fund
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2012-09-01
2024-04-26
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Bacillus eiseniae sp. nov., a swarming, moderately halotolerant bacterium isolated from the intestinal tract of an earthworm (Eisenia fetida L.)
Int J Syst Evol Microbiol 62, 2077 (2012); https://doi.org/10.1099/ijs.0.034892-0
/content/journal/ijsem/10.1099/ijs.0.034892-0
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