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Volume 63, Issue Pt_8

sp. nov., isolated from fermented rice grain ( L. subsp. ) Free

Abstract

The taxonomic position of three -like micro-organisms (strains SG293, SG296 and SG310) isolated from fermented rice grain ( L. subsp. ) in Japan was investigated. These heterofermentative lactic acid bacteria were Gram-stain-positive, rod-shaped, facultatively anaerobic, non-motile, non-spore-forming and did not show catalase activity. 16S rRNA gene sequence analysis of strain SG293 revealed that the type strains of (98.3 %), (96.2 %), (96.1 %), (96.1 %), (95.9 %) and (95.7 %) were the closest neighbours. Additional phylogenetic analysis on the basis of and gene sequences, as well as biochemical and physiological characteristics, indicated that these three strains were members of the genus and that the novel isolates had a unique taxonomic position. The predominant cellular fatty acids were Cω9 and C cyclo 9,10. Because low DNA–DNA hybridization values among the isolates and JCM 12497 were observed, it is proposed that these unidentified isolates be classified as a novel species of the genus , sp. nov. The type strain is SG293 ( = JCM 18671 = DSM 26518).

  • Published Online:
Funding
This study was supported by the:
  • Ministry of Agriculture, Forestry and Fisheries of Japan (Award 23780274)
  • Ministry of Agriculture, Forestry and Fisheries of Japan
© 2013 IUMS
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2013-08-01
2024-04-26
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Lactobacillusoryzae sp. nov., isolated from fermented rice grain (Oryza sativa L. subsp. japonica)
Int J Syst Evol Microbiol 63, 2957 (2013); https://doi.org/10.1099/ijs.0.048918-0
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