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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_11

sp. nov., isolated from a Chinese medicinal plant Free

Abstract

Two actinobacterial strains, CPCC 203464 and CPCC 203448, isolated from surface-sterilized stems of medicinal plants were subjected to a polyphasic taxonomic study. These two aerobic organisms formed pale yellow colonies on tryptic soy agar (TSA). Cells were Gram-stain-positive, non-acid-fast, non-motile, rod- or coccoid-like elements. Comparative 16S rRNA gene sequence analysis indicated that strains CPCC 203464 and CPCC 203448 were most closely related to the type strains of the species of the genus Chemotaxonomic properties such as containing diaminopimelic acid in the cell wall, arabinose, galactose and ribose being the whole-cell hydrolysate sugars, phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylinositol (PI) as the phospholipids, and C, 10-methyl C, Cω9, Cω7 and/or iso-C 2-OH as major fatty acids supported the affiliation of strains CPCC 203464 and CPCC 203448 to the genus . The DNA–DNA hybridization values in combination with differentiating chemotaxonomic and physiological characteristics strongly suggested that these two isolates should be classified as representatives of a novel species of the genus . The name sp. nov. is proposed, with strain CPCC 203464 ( = DSM 45741 = KCTC 29118) as the type strain.

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 81173026 and 31170041)
  • National Infrastructure of Microbial Resources (Award NIMR-2012-3)
  • National S&T Major Special Project on Major New Drug Innovation (Award 2012ZX09301002-003 and 2012ZX09301002-001)
  • Special Fund for Health-scientific Research in the Public Interest (Award 201002021)
© 2013 IUMS
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2013-11-01
2024-04-28
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Williamsia sterculiae sp. nov., isolated from a Chinese medicinal plant
Int J Syst Evol Microbiol 63, 4158 (2013); https://doi.org/10.1099/ijs.0.052688-0
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