1887

Abstract

A novel anaerobic succinate-producing bacterium, strain ZWB, was isolated from sludge collected from a biogas desulfurization bioreactor (Eerbeek, the Netherlands). Cells were non-spore-forming, motile, slightly curved rods (0.4–0.5 µm in diameter and 2–3 µm in length), and stained Gram-negative. The temperature range for growth was 25–40 °C, with an optimum at 37 °C. The pH range for growth was 7.0–9.0, with an optimum at pH 7.5. Strain ZWB was able to ferment glycerol and several carbohydrates mainly to H, succinate and acetate. Sulfur and fumarate could be used as electron acceptors by strain ZWB. The G+C content of the genomic DNA was 37.6 mol%. The most abundant fatty acids were iso-C and iso-C DMA. On the basis of 16S rRNA gene sequence similarity, strain ZWB belongs to the family and it is distantly related to JCM 14006 (92.1 %). Based on the physiological features and phylogenetic analysis, strain ZWB represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is ZWB ( = DSM 27333 = JCM 19283).

Funding
This study was supported by the:
  • Netherlands Ministry of Education, Culture and Science and the Netherlands Science Foundation (NWO)
  • WIMEK SENSE
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.058966-0
2014-07-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/7/2449.html?itemId=/content/journal/ijsem/10.1099/ijs.0.058966-0&mimeType=html&fmt=ahah

References

  1. Acinas S. G., Marcelino L. A., Klepac-Ceraj V., Polz M. F. ( 2004 ). Divergence and redundancy of 16S rRNA sequences in genomes with multiple rrn operons. . J Bacteriol 186, 26292635. [View Article] [PubMed]
    [Google Scholar]
  2. Alphenaar P. A., Groeneveld N., Van Aelst A. C. ( 1994 ). Scanning electron microscopical method for internal structure analysis of anaerobic granular sludge. . Micron 25, 129133. [View Article]
    [Google Scholar]
  3. Chen S., Niu L., Zhang Y. ( 2010 ). Saccharofermentans acetigenes gen. nov., sp. nov., an anaerobic bacterium isolated from sludge treating brewery wastewater. . Int J Syst Evol Microbiol 60, 27352738. [View Article] [PubMed]
    [Google Scholar]
  4. Choi W. J. ( 2008 ). Glycerol-based biorefinery for fuels and chemicals. . Recent Pat Biotechnol 2, 173180. [View Article] [PubMed]
    [Google Scholar]
  5. Cline J. D. ( 1969 ). Spectrophotometric determination of hydrogen sulfide in natural waters. . Limnol Oceanogr 14, 454458. [View Article]
    [Google Scholar]
  6. Coenye T., Vandamme P. ( 2003 ). Intragenomic heterogeneity between multiple 16S ribosomal RNA operons in sequenced bacterial genomes. . FEMS Microbiol Lett 228, 4549. [View Article] [PubMed]
    [Google Scholar]
  7. Damsté J. S., Rijpstra W. I., Hopmans E. C., Weijers J. W., Foesel B. U., Overmann J., Dedysh S. N. ( 2011 ). 13,16-Dimethyl octacosanedioic acid (iso-diabolic acid), a common membrane-spanning lipid of Acidobacteria subdivisions 1 and 3. . Appl Environ Microbiol 77, 41474154. [View Article] [PubMed]
    [Google Scholar]
  8. Doetsch R. ( 1981 ). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 2133. Edited by Gerhardt P., Murray R. D. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Philips G. B. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  9. Falsen E., Collins M. D., Welinder-Olsson C., Song Y., Finegold S. M., Lawson P. A. ( 2005 ). Fastidiosipila sanguinis gen. nov., sp. nov., a new Gram-positive, coccus-shaped organism from human blood. . Int J Syst Evol Microbiol 55, 853858. [View Article] [PubMed]
    [Google Scholar]
  10. Holdeman L. V., Cato E. P., Moore W. ( 1977 ). Anaerobe Laboratory Manual, , 4th edn.. Blacksburg:: Virginia Polytechnic Institute and State University;.
    [Google Scholar]
  11. Lee P. C., Lee W. G., Lee S. Y., Chang H. N. ( 2001 ). Succinic acid production with reduced by-product formation in the fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source. . Biotechnol Bioeng 72, 4148. [View Article] [PubMed]
    [Google Scholar]
  12. Licht F. O. ( 2012 ). World Ethanol and Biofuels Report 10, 281.
    [Google Scholar]
  13. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  14. McKinlay J. B., Vieille C., Zeikus J. G. ( 2007 ). Prospects for a bio-based succinate industry. . Appl Microbiol Biotechnol 76, 727740. [View Article] [PubMed]
    [Google Scholar]
  15. Paltauf F. ( 1994 ). Ether lipids in biomembranes. . Chem Phys Lipids 74, 101139. [View Article] [PubMed]
    [Google Scholar]
  16. Pruesse E., Peplies J., Glöckner F. O. ( 2012 ). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28, 18231829. [View Article] [PubMed]
    [Google Scholar]
  17. Röttig A., Wenning L., Bröker D., Steinbüchel A. ( 2010 ). Fatty acid alkyl esters: perspectives for production of alternative biofuels. . Appl Microbiol Biotechnol 85, 17131733. [View Article] [PubMed]
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  19. Stams A. J. M., Van Dijk J. B., Dijkema C., Plugge C. M. ( 1993 ). Growth of syntrophic propionate-oxidizing bacteria with fumarate in the absence of methanogenic bacteria. . Appl Environ Microbiol 59, 11141119.[PubMed]
    [Google Scholar]
  20. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  21. Tourova T., Kuznetzov B., Novikova E., Poltaraus A., Nazina T. ( 2001 ). [Heterogeneity of the nucleotide sequences of the 16S rRNA genes of the type strain of Desulfotomaculum kuznetsovii]. . Microbiologiia 70, 678684 [in Russian]. [View Article]
    [Google Scholar]
  22. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R. ( 2008 ). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31, 241250. [View Article] [PubMed]
    [Google Scholar]
  23. Yazdani S. S., Gonzalez R. ( 2007 ). Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. . Curr Opin Biotechnol 18, 213219. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.058966-0
Loading
/content/journal/ijsem/10.1099/ijs.0.058966-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error