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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_7

sp. nov., isolated from bamboo () litter Free

Abstract

A novel strain designated Wo-34 was isolated from bamboo () litter. Cells were Gram-stain-negative, non-motile, catalase-negative and oxidase-positive rods. The isolate grew aerobically at 15–35 °C (optimum 28 °C), pH 4.0–9.0 (optimum pH 7.0) and in the presence of 0–1.0 % (w/v) NaCl (optimum 0.1 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain Wo-34 belonged to the genus with the sequence similarities of 97.9 % and 97.3 % to the other species of the genus , 521 and KIS14-15, respectively. The predominant ubiquinone was Q-10. Major fatty acids were Cω7, C 2-OH and C cyclo ω8. The polar lipids contained phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown aminolipid, unidentified phospholipids and unknown lipids. DNA–DNA relatedness values between strain Wo-34 and DSM 23428 and KACC 13034 were 35 % and 29 %, respectively. On the basis of polyphasic analysis from this study, strain Wo-34 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is Wo-34 ( = KACC 17605 = NBRC 109813).

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Funding
This study was supported by the:
  • Regional SubGenBank Support Program of Rural Development Administration, Republic of Korea
© 2014 IUMS
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2014-07-01
2024-04-26
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References

  1. Collins M. D., Jones D. ( 1981 ). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. . Microbiol Rev 45, 316–354.[PubMed]
    [Google Scholar]
  2. DeLong E. F. ( 1992 ). Archaea in coastal marine environments. . Proc Natl Acad Sci U S A 89, 5685–5689. [View Article] [PubMed]
    [Google Scholar]
  3. Euzéby J. P. ( 2014 ). List of Prokaryotic names with Standing in Nomenclature. . http://www.bacterio.net.
     [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [View Article]
    [Google Scholar]
  5. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  7. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406–416. [View Article]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  9. Kim S.-J., Ahn J.-H., Lee T.-H., Weon H.-Y., Hong S.-B., Seok S.-J., Whang K.-S., Kwon S.-W. ( 2013 ). Reyranella soli sp. nov., isolated from forest soil, and emended description of the genus Reyranella Pagnier et al. 2011. . Int J Syst Evol Microbiol 63, 3164–3167. [View Article] [PubMed]
    [Google Scholar]
  10. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  11. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. & other authors ( 2007 ). clustal w and clustal_x version 2.0. . Bioinformatics 23, 2947–2948. [View Article] [PubMed]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  13. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  14. Murray R. G. E., Doetsch R. N., Robinow F. ( 1994 ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  15. Pagnier I., Raoult D., La Scola B. ( 2011 ). Isolation and characterization of Reyranella massiliensis gen. nov., sp. nov. from freshwater samples by using an amoeba co-culture procedure. . Int J Syst Evol Microbiol 61, 2151–2154. [View Article] [PubMed]
    [Google Scholar]
  16. Pfennig N., Trüper H. G. ( 1971 ). Higher taxa of the phototrophic bacteria. . Int J Syst Bacteriol 21, 17–18. [View Article]
    [Google Scholar]
  17. Saito H., Miura K. I. ( 1963 ). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72, 619–629. [View Article] [PubMed]
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  21. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
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Reyranellagraminifolii sp. nov., isolated from bamboo (Phyllostachys bambusoides) litter
Int J Syst Evol Microbiol 64, 2503 (2014); https://doi.org/10.1099/ijs.0.062968-0
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